Substrate interactions between toluene and methyltert-butyl ether (MTBE) during microbial degradation by Pseudomonas putida

S. G. Lee, Dong Ju Kim, J. W. Choi, Sang-Hyup Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Pseudomonas putida is well known as a toluene-degrading bacterium. Because methyl tert-butyl ether (MTBE) often coexists with toluene in subsurface environment, in this study we investigated substrate interactions between toluene and MTBE, and their degradation potential in mixtures by Pseudomonas putida. Batch microcosm studies were conducted for a wide concentration range (25-200 mg L -1) to monitor bacterial growth associated with toluene and/or MTBE degradation using Pseudomonas putida previously adapted to toluene. We found that the effect of MTBE on toluene degradation was strongly concentration-dependent, and that concentrations of either toluene or MTBE as low as 25 mg L -1 can enhance degradation of other compound, but those concentrations higher than 25 mg L -1 tend to result in negative or no effect. The negative effect was more pronounced for toluene since MTBE degradation was inhibited at a concentration of 50 mg L -1 or higher toluene. This indicates that concentrations of cocontaminants become a limiting factor for bioremediation of groundwater. Therefore, for higher degradation efficiency, care should be taken in the selection of concentration ranges of toluene and MTBE.

Original languageEnglish
Pages (from-to)278-283
Number of pages6
JournalEnvironmental Progress and Sustainable Energy
Volume30
Issue number3
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Toluene
ether
Ether
toluene
Ethers
substrate
Degradation
degradation
Substrates
MTBE
Bioremediation
bioremediation
microcosm
limiting factor
Groundwater
Bacteria
bacterium
groundwater

Keywords

  • MTBE
  • Pseudomonas putida
  • substrate interaction
  • toluene
  • toluene-degrading bacterium

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Environmental Engineering
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)

Cite this

Substrate interactions between toluene and methyltert-butyl ether (MTBE) during microbial degradation by Pseudomonas putida. / Lee, S. G.; Kim, Dong Ju; Choi, J. W.; Lee, Sang-Hyup.

In: Environmental Progress and Sustainable Energy, Vol. 30, No. 3, 01.10.2011, p. 278-283.

Research output: Contribution to journalArticle

@article{3c920470c72b4dd1a3d18839a57aa9bd,
title = "Substrate interactions between toluene and methyltert-butyl ether (MTBE) during microbial degradation by Pseudomonas putida",
abstract = "Pseudomonas putida is well known as a toluene-degrading bacterium. Because methyl tert-butyl ether (MTBE) often coexists with toluene in subsurface environment, in this study we investigated substrate interactions between toluene and MTBE, and their degradation potential in mixtures by Pseudomonas putida. Batch microcosm studies were conducted for a wide concentration range (25-200 mg L -1) to monitor bacterial growth associated with toluene and/or MTBE degradation using Pseudomonas putida previously adapted to toluene. We found that the effect of MTBE on toluene degradation was strongly concentration-dependent, and that concentrations of either toluene or MTBE as low as 25 mg L -1 can enhance degradation of other compound, but those concentrations higher than 25 mg L -1 tend to result in negative or no effect. The negative effect was more pronounced for toluene since MTBE degradation was inhibited at a concentration of 50 mg L -1 or higher toluene. This indicates that concentrations of cocontaminants become a limiting factor for bioremediation of groundwater. Therefore, for higher degradation efficiency, care should be taken in the selection of concentration ranges of toluene and MTBE.",
keywords = "MTBE, Pseudomonas putida, substrate interaction, toluene, toluene-degrading bacterium",
author = "Lee, {S. G.} and Kim, {Dong Ju} and Choi, {J. W.} and Sang-Hyup Lee",
year = "2011",
month = "10",
day = "1",
doi = "10.1002/ep.10472",
language = "English",
volume = "30",
pages = "278--283",
journal = "Environmental Progress and Sustainable Energy",
issn = "1944-7442",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Substrate interactions between toluene and methyltert-butyl ether (MTBE) during microbial degradation by Pseudomonas putida

AU - Lee, S. G.

AU - Kim, Dong Ju

AU - Choi, J. W.

AU - Lee, Sang-Hyup

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Pseudomonas putida is well known as a toluene-degrading bacterium. Because methyl tert-butyl ether (MTBE) often coexists with toluene in subsurface environment, in this study we investigated substrate interactions between toluene and MTBE, and their degradation potential in mixtures by Pseudomonas putida. Batch microcosm studies were conducted for a wide concentration range (25-200 mg L -1) to monitor bacterial growth associated with toluene and/or MTBE degradation using Pseudomonas putida previously adapted to toluene. We found that the effect of MTBE on toluene degradation was strongly concentration-dependent, and that concentrations of either toluene or MTBE as low as 25 mg L -1 can enhance degradation of other compound, but those concentrations higher than 25 mg L -1 tend to result in negative or no effect. The negative effect was more pronounced for toluene since MTBE degradation was inhibited at a concentration of 50 mg L -1 or higher toluene. This indicates that concentrations of cocontaminants become a limiting factor for bioremediation of groundwater. Therefore, for higher degradation efficiency, care should be taken in the selection of concentration ranges of toluene and MTBE.

AB - Pseudomonas putida is well known as a toluene-degrading bacterium. Because methyl tert-butyl ether (MTBE) often coexists with toluene in subsurface environment, in this study we investigated substrate interactions between toluene and MTBE, and their degradation potential in mixtures by Pseudomonas putida. Batch microcosm studies were conducted for a wide concentration range (25-200 mg L -1) to monitor bacterial growth associated with toluene and/or MTBE degradation using Pseudomonas putida previously adapted to toluene. We found that the effect of MTBE on toluene degradation was strongly concentration-dependent, and that concentrations of either toluene or MTBE as low as 25 mg L -1 can enhance degradation of other compound, but those concentrations higher than 25 mg L -1 tend to result in negative or no effect. The negative effect was more pronounced for toluene since MTBE degradation was inhibited at a concentration of 50 mg L -1 or higher toluene. This indicates that concentrations of cocontaminants become a limiting factor for bioremediation of groundwater. Therefore, for higher degradation efficiency, care should be taken in the selection of concentration ranges of toluene and MTBE.

KW - MTBE

KW - Pseudomonas putida

KW - substrate interaction

KW - toluene

KW - toluene-degrading bacterium

UR - http://www.scopus.com/inward/record.url?scp=80052948482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052948482&partnerID=8YFLogxK

U2 - 10.1002/ep.10472

DO - 10.1002/ep.10472

M3 - Article

AN - SCOPUS:80052948482

VL - 30

SP - 278

EP - 283

JO - Environmental Progress and Sustainable Energy

JF - Environmental Progress and Sustainable Energy

SN - 1944-7442

IS - 3

ER -