Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del

Hyojeong Yi, Karan Kim, Kwang Hwi Cho, Oksung Jung, Heenam Stanley Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.

Original languageEnglish
Pages (from-to)4005-4008
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume56
Issue number7
DOIs
Publication statusPublished - 2012 Jul

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del'. Together they form a unique fingerprint.

  • Cite this