Subtypes of vagal afferent C-fibres in guinea-pig lungs

Bradley J. Undem, B. Chuaychoo, M. G. Lee, D. Weinreich, A. C. Myers, M. Kollarik

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

An ex vivo, vagally innervated, lung preparation was used to address the hypothesis that vagal C-fibres comprise at least two distinct phenotypes. Histological and extracellular electrophysiological experiments revealed that vagal C-fibres innervating the pulmonary system are derived from cell bodies situated in two distinct vagal sensory ganglia. The jugular (superior) ganglion neurones project C-fibres to both the extrapulmonary airways (larynx, trachea and bronchus) and the lung parenchymal tissue. By contrast, C-fibres from nodose (inferior) neurones innervate primarily structures within the lungs. Histologically, nodose neurones projecting lung C-fibres were different from the jugular neurones in that they were significantly less likely to express neurokinins. The nerve terminals within the lungs of both nodose and jugular C-fibres responded with action potential discharge to capsaicin and bradykinin application, but only the nodose C-fibre population responded with action potential discharge to the P2X selective receptor agonist α/β-methylene-ATP. Whole cell patch clamp recording of capsaicin-sensitive nodose and jugular ganglion neurones retrogradely labelled from the lung tissue revealed that, like the nerve terminals, lung specific nodose C-fibre neurones express functional P2X receptors, whereas lung specific jugular C-fibres do not. The data support the hypothesis that both neural crest-derived neurones (jugular ganglia) and placode-derived neurones (nodose ganglia) project C-fibres in the vagus, and that these two C-fibre populations represent distinct phenotypes.

Original languageEnglish
Pages (from-to)905-917
Number of pages13
JournalJournal of Physiology
Volume556
Issue number3
DOIs
Publication statusPublished - 2004 May 1

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Subtypes of vagal afferent C-fibres in guinea-pig lungs'. Together they form a unique fingerprint.

  • Cite this

    Undem, B. J., Chuaychoo, B., Lee, M. G., Weinreich, D., Myers, A. C., & Kollarik, M. (2004). Subtypes of vagal afferent C-fibres in guinea-pig lungs. Journal of Physiology, 556(3), 905-917. https://doi.org/10.1113/jphysiol.2003.060079