Subunits of the vacuolar H+-ATPase complex, Vma4 and Vma10, are essential for virulence and represent potential drug targets in Candida albicans

Se Woong Kim, Young Kwang Park, Yoo Jin Joo, Yu Jin Chun, Ju Yeon Hwang, Je Hyun Baek, Joon Kim

Research output: Contribution to journalArticle

Abstract

Hyphal morphogenesis of Candida albicans is important for its pathogenesis. Here, we showed that the filamentous growth of C. albicans requires vacuolar H+-ATPase function. Results showed that levels of Vma4 and Vma10 increased in cells undergoing hyphal growth compared to those undergoing yeast growth. Deleting VMA4 or VMA10 abolished vacuolar functions and hyphal morphogenesis. These deletion mutants were also characterized as avirulent in a mouse model of systemic infection. Furthermore, VMA4 and VMA10 deletion strains showed hypersensitivity to fluconazole, terbinafine, and amphotericin B. Based on these findings, Vma4 and Vma10 are not only involved in vacuole biogenesis and hyphal formation, but also are good targets for antifungal drug development in C. albicans.

Original languageEnglish
JournalFungal Biology
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Vacuolar Proton-Translocating ATPases
H-transporting ATP synthase
Proton-Translocating ATPases
virulence
Candida albicans
Virulence
drug
terbinafine
morphogenesis
Morphogenesis
drugs
Growth
drug development
Pharmaceutical Preparations
antifungal agents
fluconazole
Fluconazole
amphotericin B
Amphotericin B
Vacuoles

Keywords

  • C. albicans
  • Candida drug
  • Morphogenesis
  • Pathogenesis
  • V-ATPase

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Infectious Diseases

Cite this

Subunits of the vacuolar H+-ATPase complex, Vma4 and Vma10, are essential for virulence and represent potential drug targets in Candida albicans. / Kim, Se Woong; Park, Young Kwang; Joo, Yoo Jin; Chun, Yu Jin; Hwang, Ju Yeon; Baek, Je Hyun; Kim, Joon.

In: Fungal Biology, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Kim, Se Woong

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AU - Hwang, Ju Yeon

AU - Baek, Je Hyun

AU - Kim, Joon

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