Succinate production from CO2-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing

Jungseok Lee, Sang Jun Sim, Michael Bott, Youngsoon Um, Min-Kyu Oh, Han Min Woo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The potential for production of chemicals from microalgal biomass has been considered as an alternative route for CO2 mitigation and establishment of biorefineries. This study presents the development of consolidated bioprocessing for succinate production from microalgal biomass using engineered Corynebacterium glutamicum. Starch-degrading and succinate-producing C. glutamicum strains produced succinate (0.16g succinate/g total carbon source) from a mixture of starch and glucose as a model microalgal biomass. Subsequently, the engineered C. glutamicum strains were able to produce succinate (0.28 g succinate/g of total sugars including starch) from pretreated microalgal biomass of CO2 -grown Chlamydomonas reinhardtii. For the first time, this work shows succinate production from CO2 via sequential fermentations of CO2 -grown microalgae and engineered C. glutamicum. Therefore, consolidated bioprocessing based on microalgal biomass could be useful to promote variety of biorefineries.

Original languageEnglish
Article number5819
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 2014 Jul 24

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Corynebacterium glutamicum
Succinic Acid
Biomass
Carbon
Starch
Microalgae
Chlamydomonas reinhardtii
Fermentation
Glucose

ASJC Scopus subject areas

  • General

Cite this

Succinate production from CO2-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing. / Lee, Jungseok; Sim, Sang Jun; Bott, Michael; Um, Youngsoon; Oh, Min-Kyu; Woo, Han Min.

In: Scientific Reports, Vol. 4, 5819, 24.07.2014.

Research output: Contribution to journalArticle

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