Sulfasalazine prevents T-helper 1 immune response by suppressing interleukin-12 production in macrophages

B. Y. Kang, S. W. Chung, S. Y. Im, Y. K. Choe, Tae Sung Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Interleukin-12 (IL-12) plays a pivotal role in the development of T- helper 1 (Th1) immune response, which may be involved in the pathogenesis of chronic inflammatory autoimmune disorders. In this study we investigated the effects of sulfasalazine, a drug for treating inflammatory bowel disease and rheumatoid arthritis, on the production of IL-12 from mouse macrophages stimulated with lipopolysaccharide (LPS). Sulfasalazine potently inhibited the production of IL-12 in a dose-dependent manner, in part through the down- regulation of nuclear factor κB (NFκB) activation in IL-12 p40 gene. Activation of macrophages by LPS resulted in markedly enhanced binding activities to the κB site, which significantly decreased upon addition of sulfasalazine as demonstrated by an electrophoretic gel shift assay. Importantly, macrophages pretreated with sulfasalazine either in vitro or in vivo reduced their ability to induce interferon-γ (IFN-γ) and increased the ability to induce IL-4 in antigen-primed CD4 + T cells. From these results, sulfasalazine may induce the Th2 cytokine profile in CD4 + T cells by suppressing IL-12 production in macrophages, and sulfasalazine-induced inhibition of IL-12 production in macrophages may explain some of the known biological effects of sulfasalazine.

Original languageEnglish
Pages (from-to)98-103
Number of pages6
JournalImmunology
Volume98
Issue number1
DOIs
Publication statusPublished - 1999 Sep 20
Externally publishedYes

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Sulfasalazine
Interleukin-12
Macrophages
Lipopolysaccharides
T-Lymphocytes
CD4 Antigens
Macrophage Activation
Inflammatory Bowel Diseases
Interleukin-4
Interferons
Rheumatoid Arthritis
Down-Regulation
Gels
Cytokines
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Sulfasalazine prevents T-helper 1 immune response by suppressing interleukin-12 production in macrophages. / Kang, B. Y.; Chung, S. W.; Im, S. Y.; Choe, Y. K.; Kim, Tae Sung.

In: Immunology, Vol. 98, No. 1, 20.09.1999, p. 98-103.

Research output: Contribution to journalArticle

Kang, B. Y. ; Chung, S. W. ; Im, S. Y. ; Choe, Y. K. ; Kim, Tae Sung. / Sulfasalazine prevents T-helper 1 immune response by suppressing interleukin-12 production in macrophages. In: Immunology. 1999 ; Vol. 98, No. 1. pp. 98-103.
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