TY - JOUR
T1 - Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast
AU - Choi, Kyung Mi
AU - Kim, Sorah
AU - Kim, Seahyun
AU - Lee, Hae Min
AU - Kaya, Alaattin
AU - Chun, Bok Hwan
AU - Lee, Yong Kwon
AU - Park, Tae Sik
AU - Lee, Cheol Koo
AU - Eyun, Seong Il
AU - Lee, Byung Cheon
N1 - Funding Information:
We thank Vadim N. Gladyshev (Harvard Medical School) and Christopher Hine (Harvard School of Public Health) for their valuable discussions and sharing their reagents. This work was supported by National Research Foundation of Korea (NRF) grants (2018R1A1A1 A05079386, 2018M3A9F3055925) funded by the Korean government (Ministry of Science, ICT & Future Planning) and the Korea University Future Research Grant awarded to B.C.L.
PY - 2019
Y1 - 2019
N2 - Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.
AB - Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.
KW - High-throughput genetic screening
KW - Hydrogen sulfide
KW - Methionine restriction
KW - Reactive oxygen species
KW - Sulfate assimilation
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U2 - 10.18632/aging.102050
DO - 10.18632/aging.102050
M3 - Article
C2 - 31254461
AN - SCOPUS:85068968390
VL - 11
SP - 4254
EP - 4273
JO - Aging
JF - Aging
SN - 0002-0966
IS - 12
ER -