Abstract
Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.
| Original language | English |
|---|---|
| Pages (from-to) | 4254-4273 |
| Number of pages | 20 |
| Journal | Aging |
| Volume | 11 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
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Keywords
- High-throughput genetic screening
- Hydrogen sulfide
- Methionine restriction
- Reactive oxygen species
- Sulfate assimilation
ASJC Scopus subject areas
- Ageing
- Cell Biology
Cite this
Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast. / Choi, Kyung Mi; Kim, Sorah; Kim, Seahyun; Lee, Hae Min; Kaya, Alaattin; Chun, Bok Hwan; Lee, Yong Kwon; Park, Tae Sik; Lee, Cheol-Koo; Eyun, Seong Il; Lee, Byung Cheon.
In: Aging, Vol. 11, No. 12, 01.01.2019, p. 4254-4273.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast
AU - Choi, Kyung Mi
AU - Kim, Sorah
AU - Kim, Seahyun
AU - Lee, Hae Min
AU - Kaya, Alaattin
AU - Chun, Bok Hwan
AU - Lee, Yong Kwon
AU - Park, Tae Sik
AU - Lee, Cheol-Koo
AU - Eyun, Seong Il
AU - Lee, Byung Cheon
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.
AB - Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.
KW - High-throughput genetic screening
KW - Hydrogen sulfide
KW - Methionine restriction
KW - Reactive oxygen species
KW - Sulfate assimilation
UR - http://www.scopus.com/inward/record.url?scp=85068968390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068968390&partnerID=8YFLogxK
U2 - 10.18632/aging.102050
DO - 10.18632/aging.102050
M3 - Article
C2 - 31254461
AN - SCOPUS:85068968390
VL - 11
SP - 4254
EP - 4273
JO - Aging
JF - Aging
SN - 0002-0966
IS - 12
ER -