Abstract
Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.
| Original language | English |
|---|---|
| Pages (from-to) | 235-241 |
| Number of pages | 7 |
| Journal | Nutrition |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2012 Mar 1 |
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Keywords
- Amino acid metabolism
- Brain metabolism
- Glutathione
- Nutritional assessment
- Oxidative stress
- Protein malnutrition
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Nutrition and Dietetics
Cite this
Sulfur amino acid-free diet results in increased glutamate in human midbrain : A pilot magnetic resonance spectroscopic study. / Park, Youngja; Zhao, Tiejun; Miller, Nana Gletsu; Kim, Seoung Bum; Accardi, Carolyn Jonas; Ziegler, Thomas R.; Hu, Xiaoping; Jones, Dean P.
In: Nutrition, Vol. 28, No. 3, 01.03.2012, p. 235-241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sulfur amino acid-free diet results in increased glutamate in human midbrain
T2 - A pilot magnetic resonance spectroscopic study
AU - Park, Youngja
AU - Zhao, Tiejun
AU - Miller, Nana Gletsu
AU - Kim, Seoung Bum
AU - Accardi, Carolyn Jonas
AU - Ziegler, Thomas R.
AU - Hu, Xiaoping
AU - Jones, Dean P.
PY - 2012/3/1
Y1 - 2012/3/1
N2 - Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.
AB - Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.
KW - Amino acid metabolism
KW - Brain metabolism
KW - Glutathione
KW - Nutritional assessment
KW - Oxidative stress
KW - Protein malnutrition
UR - http://www.scopus.com/inward/record.url?scp=84856483666&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856483666&partnerID=8YFLogxK
U2 - 10.1016/j.nut.2011.05.007
DO - 10.1016/j.nut.2011.05.007
M3 - Article
VL - 28
SP - 235
EP - 241
JO - Nutrition
JF - Nutrition
SN - 0899-9007
IS - 3
ER -