Sulfur amino acid-free diet results in increased glutamate in human midbrain

A pilot magnetic resonance spectroscopic study

Youngja Park, Tiejun Zhao, Nana Gletsu Miller, Seoung Bum Kim, Carolyn Jonas Accardi, Thomas R. Ziegler, Xiaoping Hu, Dean P. Jones

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalNutrition
Volume28
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

Fingerprint

Sulfur Amino Acids
Creatine
Mesencephalon
Glutamic Acid
Magnetic Resonance Spectroscopy
Diet
Parietal Lobe
Brain
Food
Nutrition Assessment
Cystine
Inositol
Principal Component Analysis
Glutamine
Methionine
Glutathione
Cysteine
Multivariate Analysis

Keywords

  • Amino acid metabolism
  • Brain metabolism
  • Glutathione
  • Nutritional assessment
  • Oxidative stress
  • Protein malnutrition

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Sulfur amino acid-free diet results in increased glutamate in human midbrain : A pilot magnetic resonance spectroscopic study. / Park, Youngja; Zhao, Tiejun; Miller, Nana Gletsu; Kim, Seoung Bum; Accardi, Carolyn Jonas; Ziegler, Thomas R.; Hu, Xiaoping; Jones, Dean P.

In: Nutrition, Vol. 28, No. 3, 01.03.2012, p. 235-241.

Research output: Contribution to journalArticle

Park, Youngja ; Zhao, Tiejun ; Miller, Nana Gletsu ; Kim, Seoung Bum ; Accardi, Carolyn Jonas ; Ziegler, Thomas R. ; Hu, Xiaoping ; Jones, Dean P. / Sulfur amino acid-free diet results in increased glutamate in human midbrain : A pilot magnetic resonance spectroscopic study. In: Nutrition. 2012 ; Vol. 28, No. 3. pp. 235-241.
@article{34139097c8c64695b38cc5ec2751f9f9,
title = "Sulfur amino acid-free diet results in increased glutamate in human midbrain: A pilot magnetic resonance spectroscopic study",
abstract = "Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.",
keywords = "Amino acid metabolism, Brain metabolism, Glutathione, Nutritional assessment, Oxidative stress, Protein malnutrition",
author = "Youngja Park and Tiejun Zhao and Miller, {Nana Gletsu} and Kim, {Seoung Bum} and Accardi, {Carolyn Jonas} and Ziegler, {Thomas R.} and Xiaoping Hu and Jones, {Dean P.}",
year = "2012",
month = "3",
day = "1",
doi = "10.1016/j.nut.2011.05.007",
language = "English",
volume = "28",
pages = "235--241",
journal = "Nutrition",
issn = "0899-9007",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Sulfur amino acid-free diet results in increased glutamate in human midbrain

T2 - A pilot magnetic resonance spectroscopic study

AU - Park, Youngja

AU - Zhao, Tiejun

AU - Miller, Nana Gletsu

AU - Kim, Seoung Bum

AU - Accardi, Carolyn Jonas

AU - Ziegler, Thomas R.

AU - Hu, Xiaoping

AU - Jones, Dean P.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.

AB - Objective: This pilot study was designed to determine if metabolic effects in different brain regions (left and right parietal lobes, midbrain) caused by 3 d of food consumption without methionine or cysteine could be detected by proton magnetic resonance spectroscopy. Methods: Healthy individuals 18 to 36 y old (n = 8) were studied by magnetic resonance spectroscopy after receiving a diet with adequate sulfur amino acids (SAAs) or with zero SAA for 3 d. Pulse sequences were used to selectively measure glutathione (GSH), and linear combination modeling of spectra was used to measure other high-abundance brain metabolites and expressed relative to creatine (Cr). Results: Although dietary SAAs are required to maintain GSH, the 3-d SAA insufficiency resulted in no significant change in GSH/Cr in the three brain regions. Principal component analysis of 16 metabolites measured by linear combination modeling showed that the metabolic pattern in the midbrain, but not in the parietal lobes, was distinguished according to the dietary SAAs. Multivariate statistical analysis showed that the major discriminating factors were signals of glutamate/Cr, (glutamate + glutamine)/Cr, and myoinositol/Cr. Correlation analyses between midbrain metabolites and GSH-related metabolites in plasma showed that midbrain glutamate/Cr had an inverse correlation with plasma cystine. Conclusion: The data show that magnetic resonance spectroscopy is a non-invasive tool suitable for nutritional assessment and suggest that nutritional imbalance caused by 3 d of SAA-free food more selectively affects the midbrain than the parietal lobes.

KW - Amino acid metabolism

KW - Brain metabolism

KW - Glutathione

KW - Nutritional assessment

KW - Oxidative stress

KW - Protein malnutrition

UR - http://www.scopus.com/inward/record.url?scp=84856483666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856483666&partnerID=8YFLogxK

U2 - 10.1016/j.nut.2011.05.007

DO - 10.1016/j.nut.2011.05.007

M3 - Article

VL - 28

SP - 235

EP - 241

JO - Nutrition

JF - Nutrition

SN - 0899-9007

IS - 3

ER -