Super‐resolution microscopy of genome organization

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)

Abstract

Recent advancements in sequencing and imaging technologies are providing new perspectives in solving the mystery of three-dimensional folding of genome in a nucleus. Chromosome conformation capture sequencing has discovered new chromatin structures such as topologically associated domains and loops in hundreds of kilobases. Super-resolution fluorescence microscopy with nanometer resolutions, in particular multiplexed approaches with sequence-specificity, has visualized chromatin structures from the rough folds of whole chromosomes to the fine loops of cis-regulatory elements in intact individual nuclei. Here, recent advancements in genome visualization tools with highly multiplexed labeling and reading are introduced. These imaging technologies have found ensemble behavior consistent to sequencing results, while unveiling single-cell variations. But, they also generated contradictory results on the roles of architectural proteins (like cohesion and CTCF) and enhancer-promoter interactions. Live-cell labeling methods for imaging specific genomic loci, especially the CRISPR/dCas9 system, are reviewed in order to give perspectives in the emergence of tools for visualizing genome structural dynamics.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalGenes and Genomics
Volume43
Issue number3
DOIs
Publication statusPublished - 2021 Mar

Keywords

  • Chromatin structure
  • Fluorescence microscopy
  • Genome architecture
  • Super‐resolution microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Super‐resolution microscopy of genome organization'. Together they form a unique fingerprint.

Cite this