Supporting data on enhanced reprogramming of human CD34+ hematopoietic stem cells to induced pluripotent stem cells using human placenta-derived cell conditioned medium

Seung Jin Lee, Ji Hea Kim, Ka Won Kang, Young Park, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The data presented herein support “Generation of an induced pluripotent stem cell line KUMCi001-A from CD34+ bone marrow cells of a patient with acute lymphoblastic leukemia using human placenta-derived cell conditioned medium.” The supplementary data were as follows. (1) Comparison of reprogramming efficiency of human placenta-derived cell conditioned medium with defined medium (mTeSR™1) and the generation of induced pluripotent stem cells (iPSCs) from a patient with acute lymphoblastic leukemia (ALL) with significantly higher reprogramming efficiency than that of the defined medium (P ≤ 0.05). (2) Evaluation of differentiation capability of the generated ALL_iPSCs into hematopoietic stem cells (HSCs) and comparison with normal iPSCs using the colony-forming unit (CFU) assay. ALL_iPSCs manifested all lineages for hematopoiesis in their colonies similar to normal iPSCs. (3) ALL_iPSCs showed a considerably higher number of burst-forming unit-erythroid colonies indicating the presence of more erythroid progenitors than normal iPSCs; this tendency was confirmed in the CFU assay of ALL_CD34+ cells. This has been previously reported as a feature of ALL. Thus, the hematopoietic characteristics of the donor patient with ALL appear to be maintained in our ALL_hiPSC line although the karyotype was normalized during reprogramming.

Original languageEnglish
Article number106140
JournalData in Brief
Volume32
DOIs
Publication statusPublished - 2020 Oct

Keywords

  • Acute lymphoblastic leukemia
  • Hematopoietic stem cells
  • Human induced pluripotent stem cells
  • Human placenta-derived cell conditioned medium
  • Reprogramming

ASJC Scopus subject areas

  • General

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