Suppression of CaCYP1, a novel cytochrome P450 gene, compromises the basal pathogen defense response of pepper plants

Young Cheol Kim, Soo Yong Kim, Kyung Hee Paek, Doil Choi, Jeong Mee Park

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A putative cytochrome P450 gene from chili pepper, Capsicum annuum L. Bukang cytochrome P450 (CaCYP1), was identified using cDNA microarray analysis of gene expression following induction of the leaf hypersensitive response by inoculation of pepper plants with the non-host pathogen Xanthomonas axonopodis pv. glycines 8ra. The full-length cDNA of CaCYP1 encoded a protein of 514 amino acid residues, which contained a putative hydrophobic membrane anchoring domain in the N-terminal region, and a heme-binding motif in the C-terminal region. Analysis of the deduced amino acid sequence of CaCYP1 revealed that it has high homology to Arabidopsis CYP89A5, the function of which is unknown. Expression of CaCYP1 was preferentially increased in pepper plants in response to non-host pathogen inoculation and also during the host resistance response. CaCYP1 expression also increased following treatment with salicylic acid and abscisic acid, while treatment with ethylene had a mild effect. Using a virus-induced gene silencing-based reverse genetics approach, we demonstrated that suppression of CaCYP1 results in enhanced susceptibility to bacterial pathogens. Interestingly, gene silencing of CaCYP1 in pepper plants resulted in the reduced expression of the defense-related genes CaLTP1, CaSIG4, and Cadhn. Our results indicated that CaCYP1, a novel cytochrome P450 in pepper plants, may play a role in plant defense response pathways that involve salicylic acid and abscisic acid signaling pathways.

Original languageEnglish
Pages (from-to)638-645
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume345
Issue number2
DOIs
Publication statusPublished - 2006 Jun 30

Fingerprint

Pathogens
Cytochrome P-450 Enzyme System
Genes
Abscisic Acid
Salicylic Acid
Capsicum
Gene Silencing
Complementary DNA
Xanthomonas axonopodis
Amino Acids
Microarrays
Reverse Genetics
Heme
Viruses
Gene expression
Glycine
Protein Sequence Analysis
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Arabidopsis

Keywords

  • Basal defense response
  • Capsicum annuum
  • Cytochrome P450
  • Disease resistance
  • Incompatible interaction
  • Non-host
  • VIGS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Suppression of CaCYP1, a novel cytochrome P450 gene, compromises the basal pathogen defense response of pepper plants. / Kim, Young Cheol; Kim, Soo Yong; Paek, Kyung Hee; Choi, Doil; Park, Jeong Mee.

In: Biochemical and Biophysical Research Communications, Vol. 345, No. 2, 30.06.2006, p. 638-645.

Research output: Contribution to journalArticle

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