Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression

Kyoung Mi Sim, Young Sun Lee, Hee Jin Kim, Chang Hoon Cho, Gwan Su Yi, Myung Jin Park, Eun Mi Hwang, Jae Yong Park

Research output: Contribution to journalArticle

Abstract

ANO1, a Ca2+-activated chloride channel, is highly expressed in glioblastoma cells and its surface expression is involved in their migration and invasion. However, the regulation of ANO1 surface expression in glioblastoma cells is largely unknown. In this study, we found that Ca2+/Calmodulin-dependent protein kinase II (CaMKII) β specifically enhances the surface expression and channel activity of ANO1 in U251 glioblastoma cells. When KN-93, a CaMKII inhibitor, was used to treat U251 cells, the surface expression and channel activity of ANO1 were significantly reduced. Only CaMKIIβ, among the four CaMKII isoforms, increased the surface expression and channel activity of ANO1 in a heterologous expression system. Additionally, gene silencing of CaMKIIβ suppressed the surface expression and channel activity of ANO1 in U251 cells. Moreover, gene silencing of CaMKIIβ or ANO1 prominently reduced the migration and invasion of U251 and U87 MG glioblastoma cells. We thus conclude that CaMKIIβ plays a specific role in the surface expression of ANO1 and in the ANO1-mediated tumorigenic properties of glioblastoma cells, such as migration and invasion.

Original languageEnglish
JournalCells
Volume9
Issue number5
DOIs
Publication statusPublished - 2020 Apr 26

Keywords

  • ANO1
  • CaMKIIβ
  • U251 glioblastoma cells
  • U87 MG glioblastoma cells

Fingerprint Dive into the research topics of 'Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression'. Together they form a unique fingerprint.

  • Cite this

    Sim, K. M., Lee, Y. S., Kim, H. J., Cho, C. H., Yi, G. S., Park, M. J., Hwang, E. M., & Park, J. Y. (2020). Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression. Cells, 9(5). https://doi.org/10.3390/cells9051079