Suppression of Ceramide-mediated Apoptosis by HSP70

Jung Hyuck Ahn, Young-Gyu Ko, Woong Yang Park, Young Sun Kang, Hee Yong Chung, Jeong Sun Seo

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Ceramide has been known as an important second messenger in programmed cell death (apoptosis) which is induced by various stimuli such as the tumor necrosis factor-α (TNF-α), Fas ligand, and environmental stresses such as UV-irradiation and heat shock. Although the precise molecular mechanism of apoptosis is not fully understood, ceramide generated by sphingomyelinase (SMase) mediates the activation of several downstream molecules that are implicated in the regulation of apoptosis. Here, we show that stress-inducible heat shock protein 70 (Hsp70) prevents apoptosis induced by increased level of intracellular ceramide. In T-cell hybridoma DO11.10, we examined the effect of Hsp70 on apoptosis mediated by TNF-α, Fas ligation, SMase, and C2-ceramide, all of which elevate intracellular ceramide levels. Hsp70 not only markedly reduced internucleosomal DNA fragmentation, but also enhanced cell viability measured by the Trypan blue dye exclusion test. Similarly, the ceramide-induced c-jun amino-terminal kinase (JNK/SAPK) activation is impaired in cells overexpressing Hsp70. These data strongly suggest that hsp70 functions as a regulator of apoptosis downstream of ceramide.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalMolecules and Cells
Volume9
Issue number2
Publication statusPublished - 1999 Apr 30
Externally publishedYes

Fingerprint

Ceramides
HSP70 Heat-Shock Proteins
Apoptosis
Sphingomyelin Phosphodiesterase
Tumor Necrosis Factor-alpha
Fas Ligand Protein
Trypan Blue
JNK Mitogen-Activated Protein Kinases
Hybridomas
Second Messenger Systems
DNA Fragmentation
Ligation
Shock
Cell Survival
Cell Death
Coloring Agents
Hot Temperature
T-Lymphocytes

Keywords

  • Apoptosis
  • Ceramide
  • Hsp70
  • JNK/SAPK

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Ahn, J. H., Ko, Y-G., Park, W. Y., Kang, Y. S., Chung, H. Y., & Seo, J. S. (1999). Suppression of Ceramide-mediated Apoptosis by HSP70. Molecules and Cells, 9(2), 200-206.

Suppression of Ceramide-mediated Apoptosis by HSP70. / Ahn, Jung Hyuck; Ko, Young-Gyu; Park, Woong Yang; Kang, Young Sun; Chung, Hee Yong; Seo, Jeong Sun.

In: Molecules and Cells, Vol. 9, No. 2, 30.04.1999, p. 200-206.

Research output: Contribution to journalArticle

Ahn, JH, Ko, Y-G, Park, WY, Kang, YS, Chung, HY & Seo, JS 1999, 'Suppression of Ceramide-mediated Apoptosis by HSP70', Molecules and Cells, vol. 9, no. 2, pp. 200-206.
Ahn JH, Ko Y-G, Park WY, Kang YS, Chung HY, Seo JS. Suppression of Ceramide-mediated Apoptosis by HSP70. Molecules and Cells. 1999 Apr 30;9(2):200-206.
Ahn, Jung Hyuck ; Ko, Young-Gyu ; Park, Woong Yang ; Kang, Young Sun ; Chung, Hee Yong ; Seo, Jeong Sun. / Suppression of Ceramide-mediated Apoptosis by HSP70. In: Molecules and Cells. 1999 ; Vol. 9, No. 2. pp. 200-206.
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