Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen

Min Kyu Yum, Mi Young Jung, Dae Ho Cho, Tae Sung Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Isoflavones are ubiquitous compounds in foods and in the environment in general. Daidzein and genistein, the best known of isoflavones, are structurally similar to 17β-estradiol and known to exert estrogenic effects. They also evidence a broad variety of biological properties, including antioxidant, anti-carcinogenic, anti-atherogenic and anti-osteoporotic activities. Previously, daidzein was reported to increase the phagocytic activity of peritoneal macrophages and splenocyte proliferation, and to inhibit nitric oxide (NO) production in macrophages. However, its potential impacts on immune response in dendritic cells (DCs), antigen-presenting cells that link innate and adaptive immunity, have yet to be clearly elucidated. In this study, we evaluated the effects of isoflavones on the maturation and activation of DCs. Isoflavones (formononetin, daidzein, equol, biochanin A, genistein) were found to differentially affect the expression of CD86, a costimulatory molecule, on lipopolysaccharide (LPS)-stimulated DCs. In particular, daidzein significantly and dose-dependently inhibited the expression levels of maturation-associated cell surface markers including CD40, costimulatory molecules (CD80, CD86), and major histocompatibility complex class II (I-A b) molecule on LPS-stimulated DCs. Daidzein also suppressed pro-inflammatory cytokine production such as IL-12p40, IL-6 and TNF-α, whereas it didn't affect IL-10 and IL-1β expression. Furthermore, daidzein enhanced endocytosis and inhibited the allo-stimulatory ability of LPS-stimulated DCs on T cells, indicating that daidzein treatment can inhibit the functional maturation of DCs. These results demonstrate that daidzein may exhibit immunosuppressive activity by inhibiting the maturation and activation of DCs.

Original languageEnglish
Pages (from-to)174-181
Number of pages8
JournalToxicology and Applied Pharmacology
Volume257
Issue number2
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Phytoestrogens
Dendritic Cells
Isoflavones
Lipopolysaccharides
Genistein
Macrophages
Molecules
Equol
Chemical activation
Interleukin-12 Subunit p40
T-cells
daidzein
Peritoneal Macrophages
Adaptive Immunity
Antigen-Presenting Cells
Immunosuppressive Agents
Endocytosis
Major Histocompatibility Complex
Interleukin-1
Innate Immunity

Keywords

  • Daidzein
  • Dendritic cell
  • Immunosuppression
  • Maturation

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Suppression of dendritic cells' maturation and functions by daidzein, a phytoestrogen. / Yum, Min Kyu; Jung, Mi Young; Cho, Dae Ho; Kim, Tae Sung.

In: Toxicology and Applied Pharmacology, Vol. 257, No. 2, 01.12.2011, p. 174-181.

Research output: Contribution to journalArticle

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