Suppression of hepatitis B virus-derived human hepatocellular carcinoma by NF-κB-inducing kinase-specific siRNA using liver-targeting liposomes

Hyun Ah Cho, In Sung Park, Tae Woo Kim, Yu Kyoung Oh, Ki Sook Yang, Jin Seok Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hepatitis B virus triggers an increase of NF-κB inducing kinase (NIK)-dependent NF-κB activation, followed by the promotion of hepatocellular carcinoma (HCC). Here, we examined the inhibitory effect of NIK-specific siRNA on NF-κB signaling and HCC. The results of this study indicated that these siRNAs suppressed HBV-derived HCC by regulating NIK activation. To exert a protective effect from degradation enzyme, cationic liposomes were contrived and modified to contain β-sitosterol glucoside to target the asialoglycoprotein receptors in liver cancer cells. The cationic dimyristoyl diacyltrimethylammonium propane liposomes were prepared by a reverse-phase evaporation method with slight modification. β-Sitosterol glucoside was added to the lipid mixture at the beginning of the liposome preparation for the purpose of liver targeting. These liposomes assisted the delivery of the siRNA to specific cells and protected it from various lyases, followed by the ultimate suppression of HCC.

Original languageEnglish
Pages (from-to)1077-1086
Number of pages10
JournalArchives of Pharmacal Research
Volume32
Issue number7
DOIs
Publication statusPublished - 2009 Jul 1

Keywords

  • Beta-sitosterol glucoside
  • DMTAP
  • Hepatitis B Virus
  • Liposomes
  • NF-κB-inducing kinase
  • SiRNA

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Organic Chemistry

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