Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium

Yee Ching Ng, Ye Won Kim, Seungbo Ryu, Ariel Lee, Jeong Su Lee, Moon Jung Song

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Human norovirus (HuNoV) is the major cause of viral foodborne diseases, but no effective medication is currently available. This study reported the anti-norovirus activity of the natural extracts of Aloe vera (AV) and Eriobotryae Folium (EF). Murine norovirus 1 (MNV-1), a surrogate virus of HuNoV, was preincubated with AV or EF extracts and then subjected to plaque assays in RAW 264.7 cells. Pretreatment of AV and EF extracts effectively suppressed the infectivity of MNV-1 with an estimated half-maximal inhibitory concentration (IC50) of 0.778 and 1.196 mg/mL, respectively. Cytotoxicity of AV and EF was not observed in RAW 264.7 cells. Anthraquinone compounds found in AV, aloin and aloe-emodin showed moderate antiviral effects. When fresh cabbage was inoculated with MNV-1 on the surface, AV and EF treatments as well as aloin and aloe-emodin at 25 °C for 30 min suppressed the MNV-1 infectivity on cabbage surface as effectively as in solution, suggesting the antiviral activity of AV and EF can be applied on food surface. These results showed AV and EF may serve as potentially effective and safe norovirus inhibitors due to their relatively low cytotoxicity, which allows them to be prepared as food-safe formulations.

Original languageEnglish
Pages (from-to)1362-1370
Number of pages9
JournalFood Control
Volume73
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

Aloe
Norovirus
Aloe vera
Phytochemicals
phytopharmaceuticals
emodin
Brassica
mice
cabbage
Antiviral Agents
cytotoxicity
extracts
pathogenicity
food surfaces
vertebrate viruses
Food
Anthraquinones
Foodborne Diseases
anthraquinones
Virus Diseases

Keywords

  • Aloe vera
  • Antiviral activity
  • Eriobotryae folium
  • Foodborne
  • Natural phytochemicals
  • Norovirus

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium. / Ng, Yee Ching; Kim, Ye Won; Ryu, Seungbo; Lee, Ariel; Lee, Jeong Su; Song, Moon Jung.

In: Food Control, Vol. 73, 01.03.2017, p. 1362-1370.

Research output: Contribution to journalArticle

Ng, Yee Ching ; Kim, Ye Won ; Ryu, Seungbo ; Lee, Ariel ; Lee, Jeong Su ; Song, Moon Jung. / Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium. In: Food Control. 2017 ; Vol. 73. pp. 1362-1370.
@article{a70b5991d1404606b32d7477ea92cac6,
title = "Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium",
abstract = "Human norovirus (HuNoV) is the major cause of viral foodborne diseases, but no effective medication is currently available. This study reported the anti-norovirus activity of the natural extracts of Aloe vera (AV) and Eriobotryae Folium (EF). Murine norovirus 1 (MNV-1), a surrogate virus of HuNoV, was preincubated with AV or EF extracts and then subjected to plaque assays in RAW 264.7 cells. Pretreatment of AV and EF extracts effectively suppressed the infectivity of MNV-1 with an estimated half-maximal inhibitory concentration (IC50) of 0.778 and 1.196 mg/mL, respectively. Cytotoxicity of AV and EF was not observed in RAW 264.7 cells. Anthraquinone compounds found in AV, aloin and aloe-emodin showed moderate antiviral effects. When fresh cabbage was inoculated with MNV-1 on the surface, AV and EF treatments as well as aloin and aloe-emodin at 25 °C for 30 min suppressed the MNV-1 infectivity on cabbage surface as effectively as in solution, suggesting the antiviral activity of AV and EF can be applied on food surface. These results showed AV and EF may serve as potentially effective and safe norovirus inhibitors due to their relatively low cytotoxicity, which allows them to be prepared as food-safe formulations.",
keywords = "Aloe vera, Antiviral activity, Eriobotryae folium, Foodborne, Natural phytochemicals, Norovirus",
author = "Ng, {Yee Ching} and Kim, {Ye Won} and Seungbo Ryu and Ariel Lee and Lee, {Jeong Su} and Song, {Moon Jung}",
year = "2017",
month = "3",
day = "1",
doi = "10.1016/j.foodcont.2016.10.051",
language = "English",
volume = "73",
pages = "1362--1370",
journal = "Food Control",
issn = "0956-7135",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium

AU - Ng, Yee Ching

AU - Kim, Ye Won

AU - Ryu, Seungbo

AU - Lee, Ariel

AU - Lee, Jeong Su

AU - Song, Moon Jung

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Human norovirus (HuNoV) is the major cause of viral foodborne diseases, but no effective medication is currently available. This study reported the anti-norovirus activity of the natural extracts of Aloe vera (AV) and Eriobotryae Folium (EF). Murine norovirus 1 (MNV-1), a surrogate virus of HuNoV, was preincubated with AV or EF extracts and then subjected to plaque assays in RAW 264.7 cells. Pretreatment of AV and EF extracts effectively suppressed the infectivity of MNV-1 with an estimated half-maximal inhibitory concentration (IC50) of 0.778 and 1.196 mg/mL, respectively. Cytotoxicity of AV and EF was not observed in RAW 264.7 cells. Anthraquinone compounds found in AV, aloin and aloe-emodin showed moderate antiviral effects. When fresh cabbage was inoculated with MNV-1 on the surface, AV and EF treatments as well as aloin and aloe-emodin at 25 °C for 30 min suppressed the MNV-1 infectivity on cabbage surface as effectively as in solution, suggesting the antiviral activity of AV and EF can be applied on food surface. These results showed AV and EF may serve as potentially effective and safe norovirus inhibitors due to their relatively low cytotoxicity, which allows them to be prepared as food-safe formulations.

AB - Human norovirus (HuNoV) is the major cause of viral foodborne diseases, but no effective medication is currently available. This study reported the anti-norovirus activity of the natural extracts of Aloe vera (AV) and Eriobotryae Folium (EF). Murine norovirus 1 (MNV-1), a surrogate virus of HuNoV, was preincubated with AV or EF extracts and then subjected to plaque assays in RAW 264.7 cells. Pretreatment of AV and EF extracts effectively suppressed the infectivity of MNV-1 with an estimated half-maximal inhibitory concentration (IC50) of 0.778 and 1.196 mg/mL, respectively. Cytotoxicity of AV and EF was not observed in RAW 264.7 cells. Anthraquinone compounds found in AV, aloin and aloe-emodin showed moderate antiviral effects. When fresh cabbage was inoculated with MNV-1 on the surface, AV and EF treatments as well as aloin and aloe-emodin at 25 °C for 30 min suppressed the MNV-1 infectivity on cabbage surface as effectively as in solution, suggesting the antiviral activity of AV and EF can be applied on food surface. These results showed AV and EF may serve as potentially effective and safe norovirus inhibitors due to their relatively low cytotoxicity, which allows them to be prepared as food-safe formulations.

KW - Aloe vera

KW - Antiviral activity

KW - Eriobotryae folium

KW - Foodborne

KW - Natural phytochemicals

KW - Norovirus

UR - http://www.scopus.com/inward/record.url?scp=85006288785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006288785&partnerID=8YFLogxK

U2 - 10.1016/j.foodcont.2016.10.051

DO - 10.1016/j.foodcont.2016.10.051

M3 - Article

VL - 73

SP - 1362

EP - 1370

JO - Food Control

JF - Food Control

SN - 0956-7135

ER -