Suppression of ras transformation by serum response factor

Jae Hong Kim, Finn Eirik Johansen, Nancy Robertson, Joseph J. Catino, Ron Prywes, C. Chandra Kumar

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE) found in the promoter regions of a number of growth factor-inducible genes, as well as muscle-specific genes. The smooth muscle α-actin promoter contains two SRE sequences that can bind to SRF. Its expression is repressed in Ras-transformed fibroblast cells and depressed in revertant cells. In this study, we demonstrate that SRF can activate α-actin expression in Ras-transformed cells and that overexpression of SRF in Ras-transformed cells can revert their transformed phenotype. The ability of SRF to bind to the SRE was required for this effect, since mutations that inhibit DNA binding abolish SRF's ability to activate α- actin expression and suppress transformation by the ras oncogene. These results show that SRF, thought to be involved in stimulation of cell growth through activation of growth factor-inducible genes, can actually have the opposite effect and suggest a novel mechanism for suppression of transformation by Ras.

Original languageEnglish
Pages (from-to)13740-13743
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number19
Publication statusPublished - 1994 May 13
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Suppression of ras transformation by serum response factor'. Together they form a unique fingerprint.

Cite this