Suppression of UDP-glycosyltransferase-coding arabidopsis thaliana UGT74E2 gene expression leads to increased resistance to psuedomonas syringae pv. tomato DC3000 infection

Hyo Jun Park, Chang Seob Kwon, Joo Yong Woo, Gil Je Lee, Young Jin Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Plants possess multiple resistance mechanisms that protect themselves against pathogen attack. To identify unknown components of the defense machinery in Arabidopsis, gene-expression changes were monitored in Arabidopsis thaliana under 18 different biotic or abiotic conditions using a DNA microarray representing approximately 25% of all Arabidopsis thaliana genes (www.genevestigator.com). Seventeen genes which are early responsive to salicylic acid (SA) treatment as well as pathogen infection were selected and their T-DNA insertion mutants were obtained from SALK institute. To elucidate the role of each gene in defense response, bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 was inoculated onto individual T-DNA insertion mutants. Four mutants exhibited decreased resistance and five mutants displayed significantly enhanced resistance against Pst DC3000-infection as measured by change in symptom development as compared to wild-type plants. Among them, member of uridin diphosphate (UDP)-glycosyltransferase (UGT) was of particular interest, since a UGT mutant (At1g05680) showed enhanced resistance to Pst-infection in Arabidopsis. In systemic acquired resistance (SAR) assay, this mutant showed enhanced activation of SAR. Also, the enhanced SAR correlated with increased expression of defenserelated gene, AtPR1. These results emphasize that the glycosylation of UGT74E2 is a part of the SA-mediated disease-resistance mechanism.

Original languageEnglish
Pages (from-to)170-182
Number of pages13
JournalPlant Pathology Journal
Volume27
Issue number2
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

glycosyltransferases
Arabidopsis thaliana
tomatoes
Pseudomonas syringae pv. tomato
gene expression
mutants
systemic acquired resistance
infection
resistance mechanisms
salicylic acid
pathogens
Arabidopsis
genes
DNA
glycosylation
signs and symptoms (plants)
disease resistance
assays

Keywords

  • Arabidopsis
  • Pseudomonas syringae
  • Salicylic acid
  • Systemic acquired resistance
  • UDP-gylcosyltransferase
  • UGT74E2

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Suppression of UDP-glycosyltransferase-coding arabidopsis thaliana UGT74E2 gene expression leads to increased resistance to psuedomonas syringae pv. tomato DC3000 infection. / Park, Hyo Jun; Kwon, Chang Seob; Woo, Joo Yong; Lee, Gil Je; Kim, Young Jin; Paek, Kyung Hee.

In: Plant Pathology Journal, Vol. 27, No. 2, 01.12.2011, p. 170-182.

Research output: Contribution to journalArticle

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