Suppressive effects of Lithospermum erythrorhizon extracts on lipopolysaccharide-induced activation of AP-1 and NF-κB via mitogen-activated protein kinase pathways in mouse macrophage cells

Yeon Han Kyu, Hwan Kwon Taek, Hoon Lee Tae, Sung Joon Lee, Sung Hoon Kim, Jiyoung Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A variety of anti-inflammatory agents have been shown to exert chemopreventive activity via targeting of transcription factors such as NF-κB and AP-1. Lithospermum erythrorhizon (LE) has long been used in traditional oriental medicine. In this study, we demonstrated the inhibitory effects of LE extracts on lipopolysaccharide (LPS)-stimulated production of inflammatory cytokines. As an underlying mechanism of inhibition, LE extracts reduced LPS-induced transactivation of AP-1 as well as NF-κB in mouse macrophage cells. Electrophoretic mobility shift assays indicated that LE extracts inhibited the DNA binding activities of AP-1 and NF-κB. In addition, phosphorylation of IκB-α protein was suppressed by LE extracts. Moreover, LE extracts inhibited c-Jun N-terminal kinase and extracellular signal-regulated signaling pathways. Our results suggest that the anti-inflammatory activity of LE extracts may be mediated by the inhibition of signal transduction pathways that normally lead to the activation of AP-1and NF-κB. These inhibitory effects may be useful for chemoprevention of cancer or other chronic inflammatory diseases.

Original languageEnglish
Pages (from-to)328-333
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume41
Issue number4
Publication statusPublished - 2008 Apr

Keywords

  • AP-1
  • Chemoprevention
  • Cytokines
  • Inflammation
  • Lithospermum erythrorhizon
  • NF-κB

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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