Suppressor of Cytokine Signaling 3 Expression Induced by Varicella-Zoster Virus Infection Results in the Modulation of Virus Replication

E. J. Choi, C. H. Lee, Ok Shin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Varicella-zoster virus (VZV) is an important viral pathogen that is responsible for causing varicella (chickenpox) and herpes zoster (shingles). VZV has been shown to suppress early anti-viral innate immune responses, but the exact mechanisms are not yet well understood. Here we demonstrate that host control of VZV is impaired by the expression of suppressor of cytokine signaling (SOCS)3. We used three different cell types to characterize VZV-induced anti-viral and inflammatory responses. Infection of human fibroblasts (MRC-5) and human macrophages (THP-1) with VZV triggered upregulation of anti-viral responsive gene expression (IFN-α, IFN-β) in the early phases of infection, followed by the waning of these IFNs in the late phases of infection. Conversely, VZV infection in keratinocytes (HaCaT) resulted in a persistent increase in type I IFN gene expression. Interestingly, increase in SOCS1 and 3 expressions coincided with a reduction in phosphorylation of the signal transducer and activator of transcription protein 3 (STAT3) in VZV-infected MRC-5 cells. Furthermore, VZV infection increased the production of pro-inflammatory cytokines, including interleukin (IL)-6, -8, and IFN-γ-inducible protein 10 (IP-10). Knockdown of SOCS3 inhibited viral replication and enhanced secretion levels of IL-6, whereas overexpression of SOCS3 did not affect viral replication efficiency and host response. In conclusion, our data suggest that VZV infection induces SOCS3 expression, resulting in modulation of type I IFN signaling and viral replication.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalScandinavian Journal of Immunology
Volume82
Issue number4
DOIs
Publication statusPublished - 2015 Oct 1

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Human Herpesvirus 3
Virus Diseases
Virus Replication
Cytokines
Chickenpox
Herpes Zoster
Interleukin-6
Infection
STAT Transcription Factors
Gene Expression
STAT3 Transcription Factor
Viral Genes
Interleukin-8
Keratinocytes
Innate Immunity
Anti-Inflammatory Agents
Up-Regulation
Fibroblasts
Macrophages
Phosphorylation

ASJC Scopus subject areas

  • Immunology

Cite this

Suppressor of Cytokine Signaling 3 Expression Induced by Varicella-Zoster Virus Infection Results in the Modulation of Virus Replication. / Choi, E. J.; Lee, C. H.; Shin, Ok.

In: Scandinavian Journal of Immunology, Vol. 82, No. 4, 01.10.2015, p. 337-344.

Research output: Contribution to journalArticle

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