Surface characteristics and fibroblast adhesion behavior of RGD-immobilized biodegradable PLLA films

Jung Jung Hyun, Kwang Duk Ahn, Keun Han Dong, Dong June Ahn

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLA-g-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/cm2. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control < PLLA-g-PAA=PLLA-g-GRDG < PLLA-g-GRGD, indicating that PLLA-g-GRGD has the highest cell adhesive property.

Original languageEnglish
Pages (from-to)446-452
Number of pages7
JournalMacromolecular Research
Volume13
Issue number5
Publication statusPublished - 2005 Oct 1

Keywords

  • Direct AA grafting
  • Fibroblast adhesion
  • Plasma treatment
  • PLLA film
  • RGD immobilization
  • Tissue engineering

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry

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