Surface characteristics and fibroblast adhesion behavior of RGD-immobilized biodegradable PLLA films

Jung Jung Hyun, Kwang Duk Ahn, Keun Han Dong, Dong June Ahn

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLA-g-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/cm2. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control < PLLA-g-PAA=PLLA-g-GRDG < PLLA-g-GRGD, indicating that PLLA-g-GRGD has the highest cell adhesive property.

Original languageEnglish
Pages (from-to)446-452
Number of pages7
JournalMacromolecular Research
Volume13
Issue number5
DOIs
Publication statusPublished - 2005 Oct

Keywords

  • Direct AA grafting
  • Fibroblast adhesion
  • PLLA film
  • Plasma treatment
  • RGD immobilization
  • Tissue engineering

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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