Surface chemistry modification of PDMS elastomers with boiling water improves cellular adhesion

Joong Yull Park; Dongchan Ahn; Yoon Young Choi; Chang Mo Hwang; Shuichi Takayama; Soon Hyuck Lee; Sang Hoon Lee

doi:10.1016/j.snb.2012.06.096

Surface chemistry modification of PDMS elastomers with boiling water improves cellular adhesion

Joong Yull Park, Dongchan Ahn, Yoon Young Choi, Chang Mo Hwang, Shuichi Takayama, Soon Hyuck Lee, Sang Hoon Lee

Department of Bio-convergence Engineering

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Polydimethylsiloxane (PDMS) lacks cell binding sites and requires additional treatments such as a routine extracellular matrix coating process to culture cells on it. Here we propose a simple method to improve the cytophilicity of hydrosilylation cured PDMS elastomers by using boiling water; placing the crosslinked PDMS substrate in boiling deionized water for about 1 h generates hydroxyl groups on the surface derived from residual SiH functional groups present in the cured elastomers that act as good binding sites for cell attachment. Modified chemistry of the PDMS surface was confirmed by using attenuated total reflection infrared (ATR-IR) analysis. The beneficial biological effects were demonstrated through enhanced attachment/spreading of human mesenchymal stem cells on the boiling-treated surface of PDMS. The results show that boiling water treatment offers a mild, convenient, and cost-effective method to improve the surface cytophilicity of PDMS.

Original language	English
Pages (from-to)	765-771
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	173
DOIs	https://doi.org/10.1016/j.snb.2012.06.096
Publication status	Published - 2012 Oct

Keywords

Biocompatibility
Boiling water
Hydrophilic surface modification
Hydrosilylation
Hydroxyl groups
PDMS
Silicone elastomer

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

10.1016/j.snb.2012.06.096

Cite this

@article{075aaebccc91418faabe6734762093ea,

title = "Surface chemistry modification of PDMS elastomers with boiling water improves cellular adhesion",

abstract = "Polydimethylsiloxane (PDMS) lacks cell binding sites and requires additional treatments such as a routine extracellular matrix coating process to culture cells on it. Here we propose a simple method to improve the cytophilicity of hydrosilylation cured PDMS elastomers by using boiling water; placing the crosslinked PDMS substrate in boiling deionized water for about 1 h generates hydroxyl groups on the surface derived from residual SiH functional groups present in the cured elastomers that act as good binding sites for cell attachment. Modified chemistry of the PDMS surface was confirmed by using attenuated total reflection infrared (ATR-IR) analysis. The beneficial biological effects were demonstrated through enhanced attachment/spreading of human mesenchymal stem cells on the boiling-treated surface of PDMS. The results show that boiling water treatment offers a mild, convenient, and cost-effective method to improve the surface cytophilicity of PDMS.",

keywords = "Biocompatibility, Boiling water, Hydrophilic surface modification, Hydrosilylation, Hydroxyl groups, PDMS, Silicone elastomer",

author = "Park, {Joong Yull} and Dongchan Ahn and Choi, {Yoon Young} and Hwang, {Chang Mo} and Shuichi Takayama and Lee, {Soon Hyuck} and Lee, {Sang Hoon}",

note = "Funding Information: We thank Dr. Christopher McMillan of Dow Corning Corporation for contributing XPS characterization. We further thank Dr. Ronald Tecklenberg and Mr. Gary Kozerski of Dow Corning Corporation for helpful discussions regarding the analysis and interpretation of MALDI-TOF spectra. This research was supported by a grant of the National Research Lab (NRL) program, the Korea Science and Engineering Foundation (KOSEF), Republic of Korea ( R0A-20110020455 ), and also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012R1A1A1015181 ). ",

year = "2012",

month = oct,

doi = "10.1016/j.snb.2012.06.096",

language = "English",

volume = "173",

pages = "765--771",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

}

TY - JOUR

T1 - Surface chemistry modification of PDMS elastomers with boiling water improves cellular adhesion

AU - Park, Joong Yull

AU - Ahn, Dongchan

AU - Choi, Yoon Young

AU - Hwang, Chang Mo

AU - Takayama, Shuichi

AU - Lee, Soon Hyuck

AU - Lee, Sang Hoon

N1 - Funding Information: We thank Dr. Christopher McMillan of Dow Corning Corporation for contributing XPS characterization. We further thank Dr. Ronald Tecklenberg and Mr. Gary Kozerski of Dow Corning Corporation for helpful discussions regarding the analysis and interpretation of MALDI-TOF spectra. This research was supported by a grant of the National Research Lab (NRL) program, the Korea Science and Engineering Foundation (KOSEF), Republic of Korea ( R0A-20110020455 ), and also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2012R1A1A1015181 ).

PY - 2012/10

Y1 - 2012/10

N2 - Polydimethylsiloxane (PDMS) lacks cell binding sites and requires additional treatments such as a routine extracellular matrix coating process to culture cells on it. Here we propose a simple method to improve the cytophilicity of hydrosilylation cured PDMS elastomers by using boiling water; placing the crosslinked PDMS substrate in boiling deionized water for about 1 h generates hydroxyl groups on the surface derived from residual SiH functional groups present in the cured elastomers that act as good binding sites for cell attachment. Modified chemistry of the PDMS surface was confirmed by using attenuated total reflection infrared (ATR-IR) analysis. The beneficial biological effects were demonstrated through enhanced attachment/spreading of human mesenchymal stem cells on the boiling-treated surface of PDMS. The results show that boiling water treatment offers a mild, convenient, and cost-effective method to improve the surface cytophilicity of PDMS.

AB - Polydimethylsiloxane (PDMS) lacks cell binding sites and requires additional treatments such as a routine extracellular matrix coating process to culture cells on it. Here we propose a simple method to improve the cytophilicity of hydrosilylation cured PDMS elastomers by using boiling water; placing the crosslinked PDMS substrate in boiling deionized water for about 1 h generates hydroxyl groups on the surface derived from residual SiH functional groups present in the cured elastomers that act as good binding sites for cell attachment. Modified chemistry of the PDMS surface was confirmed by using attenuated total reflection infrared (ATR-IR) analysis. The beneficial biological effects were demonstrated through enhanced attachment/spreading of human mesenchymal stem cells on the boiling-treated surface of PDMS. The results show that boiling water treatment offers a mild, convenient, and cost-effective method to improve the surface cytophilicity of PDMS.

KW - Biocompatibility

KW - Boiling water

KW - Hydrophilic surface modification

KW - Hydrosilylation

KW - Hydroxyl groups

KW - PDMS

KW - Silicone elastomer

UR - http://www.scopus.com/inward/record.url?scp=84867075916&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2012.06.096

DO - 10.1016/j.snb.2012.06.096

M3 - Article

AN - SCOPUS:84867075916

VL - 173

SP - 765

EP - 771

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -

Surface chemistry modification of PDMS elastomers with boiling water improves cellular adhesion

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this