Surface immobilization of biphasic calcium phosphate nanoparticles on 3D printed poly(caprolactone) scaffolds enhances osteogenesis and bone tissue regeneration

Kyu Sik Shim; Sung Eun Kim; Young Pil Yun; Daniel I. Jeon; Hak Jun Kim; Kyeongsoon Park; Hae Ryong Song

doi:10.1016/j.jiec.2017.06.033

Surface immobilization of biphasic calcium phosphate nanoparticles on 3D printed poly(caprolactone) scaffolds enhances osteogenesis and bone tissue regeneration

Kyu Sik Shim, Sung Eun Kim, Young Pil Yun, Daniel I. Jeon, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We fabricated biphasic calcium phosphate nanoparticles (BCP NPs)-immobilized on the surface of 3D printed PCL (BCP-IM-PCL) scaffolds, and evaluated in vitro osteogenesis and in vivo new bone formation in rat tibial defect model. In vitro and in vivo studies showed that BCP-IM-PCL significantly enhanced osteogenic markers (i.e., ALP activity, calcium deposition, and the expression of osteocalcin and osteopontin) and markedly increased new bone formation and mineralized bone tissues in tibial defect area, compared to unmodified PCL and BCP-mixed PCL scaffolds. This study demonstrated that BCP NPs-immobilized on the surface of PCL scaffolds are promising templates for bone tissue regeneration.

Original language	English
Pages (from-to)	101-109
Number of pages	9
Journal	Journal of Industrial and Engineering Chemistry
Volume	55
DOIs	https://doi.org/10.1016/j.jiec.2017.06.033
Publication status	Published - 2017 Nov 25

Keywords

Biphasic calcium phosphate (BCP)
MG-63 cells
New bone formation
Three-dimensional (3D) printed scaffolds
Tibial defect model

ASJC Scopus subject areas

Chemical Engineering(all)

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10.1016/j.jiec.2017.06.033

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title = "Surface immobilization of biphasic calcium phosphate nanoparticles on 3D printed poly(caprolactone) scaffolds enhances osteogenesis and bone tissue regeneration",

abstract = "We fabricated biphasic calcium phosphate nanoparticles (BCP NPs)-immobilized on the surface of 3D printed PCL (BCP-IM-PCL) scaffolds, and evaluated in vitro osteogenesis and in vivo new bone formation in rat tibial defect model. In vitro and in vivo studies showed that BCP-IM-PCL significantly enhanced osteogenic markers (i.e., ALP activity, calcium deposition, and the expression of osteocalcin and osteopontin) and markedly increased new bone formation and mineralized bone tissues in tibial defect area, compared to unmodified PCL and BCP-mixed PCL scaffolds. This study demonstrated that BCP NPs-immobilized on the surface of PCL scaffolds are promising templates for bone tissue regeneration.",

keywords = "Biphasic calcium phosphate (BCP), MG-63 cells, New bone formation, Three-dimensional (3D) printed scaffolds, Tibial defect model",

author = "Shim, {Kyu Sik} and Kim, {Sung Eun} and Yun, {Young Pil} and Jeon, {Daniel I.} and Kim, {Hak Jun} and Kyeongsoon Park and Song, {Hae Ryong}",

note = "Funding Information: This study was financially supported by grants from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI11C0388). Publisher Copyright: {\textcopyright} 2017 The Korean Society of Industrial and Engineering Chemistry",

year = "2017",

month = nov,

day = "25",

doi = "10.1016/j.jiec.2017.06.033",

language = "English",

volume = "55",

pages = "101--109",

journal = "Journal of Industrial and Engineering Chemistry",

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publisher = "Korean Society of Industrial Engineering Chemistry",

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T1 - Surface immobilization of biphasic calcium phosphate nanoparticles on 3D printed poly(caprolactone) scaffolds enhances osteogenesis and bone tissue regeneration

AU - Shim, Kyu Sik

AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Jeon, Daniel I.

AU - Kim, Hak Jun

AU - Park, Kyeongsoon

AU - Song, Hae Ryong

N1 - Funding Information: This study was financially supported by grants from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI11C0388). Publisher Copyright: © 2017 The Korean Society of Industrial and Engineering Chemistry

PY - 2017/11/25

Y1 - 2017/11/25

N2 - We fabricated biphasic calcium phosphate nanoparticles (BCP NPs)-immobilized on the surface of 3D printed PCL (BCP-IM-PCL) scaffolds, and evaluated in vitro osteogenesis and in vivo new bone formation in rat tibial defect model. In vitro and in vivo studies showed that BCP-IM-PCL significantly enhanced osteogenic markers (i.e., ALP activity, calcium deposition, and the expression of osteocalcin and osteopontin) and markedly increased new bone formation and mineralized bone tissues in tibial defect area, compared to unmodified PCL and BCP-mixed PCL scaffolds. This study demonstrated that BCP NPs-immobilized on the surface of PCL scaffolds are promising templates for bone tissue regeneration.

AB - We fabricated biphasic calcium phosphate nanoparticles (BCP NPs)-immobilized on the surface of 3D printed PCL (BCP-IM-PCL) scaffolds, and evaluated in vitro osteogenesis and in vivo new bone formation in rat tibial defect model. In vitro and in vivo studies showed that BCP-IM-PCL significantly enhanced osteogenic markers (i.e., ALP activity, calcium deposition, and the expression of osteocalcin and osteopontin) and markedly increased new bone formation and mineralized bone tissues in tibial defect area, compared to unmodified PCL and BCP-mixed PCL scaffolds. This study demonstrated that BCP NPs-immobilized on the surface of PCL scaffolds are promising templates for bone tissue regeneration.

KW - Biphasic calcium phosphate (BCP)

KW - MG-63 cells

KW - New bone formation

KW - Three-dimensional (3D) printed scaffolds

KW - Tibial defect model

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U2 - 10.1016/j.jiec.2017.06.033

DO - 10.1016/j.jiec.2017.06.033

M3 - Article

AN - SCOPUS:85022218318

SN - 1226-086X

VL - 55

SP - 101

EP - 109

JO - Journal of Industrial and Engineering Chemistry

JF - Journal of Industrial and Engineering Chemistry

ER -

Surface immobilization of biphasic calcium phosphate nanoparticles on 3D printed poly(caprolactone) scaffolds enhances osteogenesis and bone tissue regeneration

Abstract

Keywords

ASJC Scopus subject areas

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