Abstract
Cationic liposomes for cancer treatment have been developed in the field of chemotharpy. It was well combined on the surface of anionic tumor cell membrane by electrostatic interaction. Thus, the object of this study was to prepare the cationic liposomes capable of forming an ionic complex with the anionic cell membrane. To prepare the cationic liposomes, 1,2-distearoyl-sn-glycero-3- phosphoethanolamine (DSPE) as a cationic lipid material and polyethylenimine (PEI) as a cationic polymer were synthesized. Ionic property on the surface of liposomes was determined by the zeta potential. The adsorption characteristics of plasma protein for liposome in bovine serum were determined by the particle size and turbidity change. To estimate the stability of liposome in buffered solution, the change of particle size was measured at room temperature for seven days. The cationic liposomes were absorbed a large amount of plasma protein in bovine serum because plasma protein having anionic charge was fixed on the surface of cationic liposomes. This result indicate that the modification on the surface of liposomes using cationic polyethylenimine enhances the protein adsorption in bovine serum. Additionaly, cationic liposomes showed good stability in buffered solution for seven days.
| Original language | English |
|---|---|
| Pages (from-to) | 502-508 |
| Number of pages | 7 |
| Journal | Polymer (Korea) |
| Volume | 28 |
| Issue number | 6 |
| Publication status | Published - 2004 Nov 1 |
| Externally published | Yes |
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Keywords
- Cationic liposomes
- Poly(ethylenimine)
- Protein adsorption
- Stability
ASJC Scopus subject areas
- Polymers and Plastics
- Materials Chemistry
Cite this
Surface properties of liposomes modified with poly(ethylenimine). / Yun, Jung Park; Da, Eun Nam; Dong, Hoan Seo; Hee, Dong Han; Kim, Tae Woo; Moon, Suk Kim; Shin, Byung Cheol.
In: Polymer (Korea), Vol. 28, No. 6, 01.11.2004, p. 502-508.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface properties of liposomes modified with poly(ethylenimine)
AU - Yun, Jung Park
AU - Da, Eun Nam
AU - Dong, Hoan Seo
AU - Hee, Dong Han
AU - Kim, Tae Woo
AU - Moon, Suk Kim
AU - Shin, Byung Cheol
PY - 2004/11/1
Y1 - 2004/11/1
N2 - Cationic liposomes for cancer treatment have been developed in the field of chemotharpy. It was well combined on the surface of anionic tumor cell membrane by electrostatic interaction. Thus, the object of this study was to prepare the cationic liposomes capable of forming an ionic complex with the anionic cell membrane. To prepare the cationic liposomes, 1,2-distearoyl-sn-glycero-3- phosphoethanolamine (DSPE) as a cationic lipid material and polyethylenimine (PEI) as a cationic polymer were synthesized. Ionic property on the surface of liposomes was determined by the zeta potential. The adsorption characteristics of plasma protein for liposome in bovine serum were determined by the particle size and turbidity change. To estimate the stability of liposome in buffered solution, the change of particle size was measured at room temperature for seven days. The cationic liposomes were absorbed a large amount of plasma protein in bovine serum because plasma protein having anionic charge was fixed on the surface of cationic liposomes. This result indicate that the modification on the surface of liposomes using cationic polyethylenimine enhances the protein adsorption in bovine serum. Additionaly, cationic liposomes showed good stability in buffered solution for seven days.
AB - Cationic liposomes for cancer treatment have been developed in the field of chemotharpy. It was well combined on the surface of anionic tumor cell membrane by electrostatic interaction. Thus, the object of this study was to prepare the cationic liposomes capable of forming an ionic complex with the anionic cell membrane. To prepare the cationic liposomes, 1,2-distearoyl-sn-glycero-3- phosphoethanolamine (DSPE) as a cationic lipid material and polyethylenimine (PEI) as a cationic polymer were synthesized. Ionic property on the surface of liposomes was determined by the zeta potential. The adsorption characteristics of plasma protein for liposome in bovine serum were determined by the particle size and turbidity change. To estimate the stability of liposome in buffered solution, the change of particle size was measured at room temperature for seven days. The cationic liposomes were absorbed a large amount of plasma protein in bovine serum because plasma protein having anionic charge was fixed on the surface of cationic liposomes. This result indicate that the modification on the surface of liposomes using cationic polyethylenimine enhances the protein adsorption in bovine serum. Additionaly, cationic liposomes showed good stability in buffered solution for seven days.
KW - Cationic liposomes
KW - Poly(ethylenimine)
KW - Protein adsorption
KW - Stability
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UR - http://www.scopus.com/inward/citedby.url?scp=24744434363&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:24744434363
VL - 28
SP - 502
EP - 508
JO - Polymer (Korea)
JF - Polymer (Korea)
SN - 0379-153X
IS - 6
ER -