Abstract
The surface modification of poly(dimethylsiloxane) (PDMS) substrates by using ABA-type block copolymers comprising poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and PDMS segments was investigated. The hydrophobic interaction between the swelling-deswelling nature of PDMS and PDMS segments in block copolymers was the main mechanism for surface modification. Block copolymers with various compositions were synthesized by using the atom transfer radical polymerization (ATRP) method. The kinetic plots revealed that polymerization could be initiated by PDMS macroinitiators and it proceeds in a well-controlled manner; therefore, the compositions of the block copolymers were controllable. The obtained block copolymers were dissolved in a chloroform/ethanol mixed solvent. The surface of the PDMS substrate was modified using block copolymers by the swelling-deswelling method. Static contact angle and X-ray photoelectron spectroscopy (XPS) measurements revealed that the hydrophobic surface of the PDMS substrate was converted to a hydrophilic surface because of modification by surface-tethered PMPC segments. Protein adsorption test and L929 cell adhesion test were carried out for evaluating the biocompatibility. As observed, the amount of adsorbed proteins and cell adhesion were drastically reduced as compared to those in the non-treated PDMS substrate. We conclude that this procedure is effective in fabricating biocompatible surfaces on PDMS substrates.
| Original language | English |
|---|---|
| Pages (from-to) | 1367-1376 |
| Number of pages | 10 |
| Journal | Biomaterials |
| Volume | 29 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2008 Apr 1 |
| Externally published | Yes |
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Keywords
- 2-Methacryloyloxyethyl phosphorylcholine
- Atom transfer radical polymerization
- Biocompatibility
- Poly(dimethylsiloxane)
- Swelling-deswelling method
ASJC Scopus subject areas
- Bioengineering
- Ceramics and Composites
- Biophysics
- Biomaterials
- Mechanics of Materials
Cite this
Surface tethering of phosphorylcholine groups onto poly(dimethylsiloxane) through swelling-deswelling methods with phospholipids moiety containing ABA-type block copolymers. / Seo, Ji-Hun; Matsuno, Ryosuke; Konno, Tomohiro; Takai, Madoka; Ishihara, Kazuhiko.
In: Biomaterials, Vol. 29, No. 10, 01.04.2008, p. 1367-1376.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface tethering of phosphorylcholine groups onto poly(dimethylsiloxane) through swelling-deswelling methods with phospholipids moiety containing ABA-type block copolymers
AU - Seo, Ji-Hun
AU - Matsuno, Ryosuke
AU - Konno, Tomohiro
AU - Takai, Madoka
AU - Ishihara, Kazuhiko
PY - 2008/4/1
Y1 - 2008/4/1
N2 - The surface modification of poly(dimethylsiloxane) (PDMS) substrates by using ABA-type block copolymers comprising poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and PDMS segments was investigated. The hydrophobic interaction between the swelling-deswelling nature of PDMS and PDMS segments in block copolymers was the main mechanism for surface modification. Block copolymers with various compositions were synthesized by using the atom transfer radical polymerization (ATRP) method. The kinetic plots revealed that polymerization could be initiated by PDMS macroinitiators and it proceeds in a well-controlled manner; therefore, the compositions of the block copolymers were controllable. The obtained block copolymers were dissolved in a chloroform/ethanol mixed solvent. The surface of the PDMS substrate was modified using block copolymers by the swelling-deswelling method. Static contact angle and X-ray photoelectron spectroscopy (XPS) measurements revealed that the hydrophobic surface of the PDMS substrate was converted to a hydrophilic surface because of modification by surface-tethered PMPC segments. Protein adsorption test and L929 cell adhesion test were carried out for evaluating the biocompatibility. As observed, the amount of adsorbed proteins and cell adhesion were drastically reduced as compared to those in the non-treated PDMS substrate. We conclude that this procedure is effective in fabricating biocompatible surfaces on PDMS substrates.
AB - The surface modification of poly(dimethylsiloxane) (PDMS) substrates by using ABA-type block copolymers comprising poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and PDMS segments was investigated. The hydrophobic interaction between the swelling-deswelling nature of PDMS and PDMS segments in block copolymers was the main mechanism for surface modification. Block copolymers with various compositions were synthesized by using the atom transfer radical polymerization (ATRP) method. The kinetic plots revealed that polymerization could be initiated by PDMS macroinitiators and it proceeds in a well-controlled manner; therefore, the compositions of the block copolymers were controllable. The obtained block copolymers were dissolved in a chloroform/ethanol mixed solvent. The surface of the PDMS substrate was modified using block copolymers by the swelling-deswelling method. Static contact angle and X-ray photoelectron spectroscopy (XPS) measurements revealed that the hydrophobic surface of the PDMS substrate was converted to a hydrophilic surface because of modification by surface-tethered PMPC segments. Protein adsorption test and L929 cell adhesion test were carried out for evaluating the biocompatibility. As observed, the amount of adsorbed proteins and cell adhesion were drastically reduced as compared to those in the non-treated PDMS substrate. We conclude that this procedure is effective in fabricating biocompatible surfaces on PDMS substrates.
KW - 2-Methacryloyloxyethyl phosphorylcholine
KW - Atom transfer radical polymerization
KW - Biocompatibility
KW - Poly(dimethylsiloxane)
KW - Swelling-deswelling method
UR - http://www.scopus.com/inward/record.url?scp=38349108126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38349108126&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2007.11.039
DO - 10.1016/j.biomaterials.2007.11.039
M3 - Article
C2 - 18155763
AN - SCOPUS:38349108126
VL - 29
SP - 1367
EP - 1376
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 10
ER -