Survey of rice proteins interacting with OsFCA and OsFY proteins which are homologous to the arabidopsis flowering time proteins, FCA and FY

Yun Hee Jang, Hyo Young Park, Soon Kap Kim, Jeong Hwan Lee, Mi Chung Suh, Young Soo Chung, Kyung Hee Paek, Jeong Kook Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The FCA protein is involved in controlling flowering time and plays more general roles in RNA-mediated chromatin silencing in Arabidopsis. It contains two RNA-binding domains and a WW domain. The FCA protein interacts with FY, a polyadenylation factor, via its WW domain. We previously characterized a rice gene, OsFCA, which was homologous to FCA. Here, we found that the OsFCA protein could interact through its WW domain with the following proteins: OsFY, a protein containing a CID domain present in RNA-processing factors such as Pcf11 and Nrd1; a protein similar to splicing factor SF1; a protein similar to FUSE splicing factor; and OsMADS8. The FY protein is associated with the 3′ end processing machinery in Arabidopsis. Thus, we examined interactions between OsFY and the rice homologs (OsCstF-50, -64 and -77) of the AtCstF-50, -64 and -77 proteins. We found that OsFY could bind OsCstF50, whereas the OsCstF77 protein could bridge the interaction between OsCstF50 and OsCstF64. Taken together, our data suggest that OsFCA could interact with several proteins other than OsFY through its WW domain and may play several roles in rice.

Original languageEnglish
Pages (from-to)1479-1492
Number of pages14
JournalPlant and Cell Physiology
Volume50
Issue number8
DOIs
Publication statusPublished - 2009 Aug

Keywords

  • Flowering time
  • OsFCA
  • OsFY
  • Polyadenylation
  • Protein interaction
  • Rice

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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