Survival of freeze-dried Lactobacillus bulgaricus KFRI 673 in chitosan-coated calcium alginate microparticles

J. S. Lee, D. S. Cha, Hyun Jin Park

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

The aim of this study was to investigate the effect of alginate microparticles coated with three kinds of chitosans of different molecular weights on the survival of Lactobacillus bulgaricus KFRI 673 in simulated gastric (SGJ) and intestinal juices (SIJ) and on their stability during storage at 4 and 22 °C. L. bulgaricus KFRI 673 loaded in alginate microparticles was prepared by spraying the mixture of sodium alginate and cell culture into the calcium chloride solution using an air-atomizing device. When L. bulgaricus KFRI 673 was exposed to SGJ of pH 2.0 for 60 min, none of the microorganism survived. Contrary to this result, microbiological analysis indicated that microencapsulation in alginate microparticles improved the survival of acid-sensitive L. bulgaricus KFRI 673 in SGJ and that high molecular weight chitosan coating resulted in the highest survival in SGJ. To study storage stability of free and microencapsulated cells, in vitro studies were conducted at 4 and 22 °C during a 4 week period. Both free and microencapsulated cells showed similar stabilities during 4 weeks of storage at 4 °C. However, the stability of Lactobacillus at 22 °C was appreciably improved when loaded in high molecular weight chitosan-coated alginate microparticles. In conclusion, microencapsulation of lactic acid bacteria with alginate and chitosan coating offers an effective way of delivering viable bacterial cells to the colon and maintaining their survival during refrigerated storage.

Original languageEnglish
Pages (from-to)7300-7305
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number24
DOIs
Publication statusPublished - 2004 Dec 1
Externally publishedYes

Fingerprint

Lactobacillus delbrueckii
calcium alginate
Lactobacillus delbrueckii subsp. bulgaricus
Chitosan
alginates
chitosan
microencapsulation
Microencapsulation
molecular weight
Drug Compounding
Molecular Weight
Molecular weight
coatings
gastric juice
sodium alginate
cells
Coatings
Calcium Chloride
in vitro studies
calcium chloride

Keywords

  • Alginate
  • Chitosan coating
  • Lactobacillus bulgaricus
  • Microparticles
  • Storage stability

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Survival of freeze-dried Lactobacillus bulgaricus KFRI 673 in chitosan-coated calcium alginate microparticles. / Lee, J. S.; Cha, D. S.; Park, Hyun Jin.

In: Journal of Agricultural and Food Chemistry, Vol. 52, No. 24, 01.12.2004, p. 7300-7305.

Research output: Contribution to journalArticle

@article{529ef2806d4a4eb6a038bc442e05847d,
title = "Survival of freeze-dried Lactobacillus bulgaricus KFRI 673 in chitosan-coated calcium alginate microparticles",
abstract = "The aim of this study was to investigate the effect of alginate microparticles coated with three kinds of chitosans of different molecular weights on the survival of Lactobacillus bulgaricus KFRI 673 in simulated gastric (SGJ) and intestinal juices (SIJ) and on their stability during storage at 4 and 22 °C. L. bulgaricus KFRI 673 loaded in alginate microparticles was prepared by spraying the mixture of sodium alginate and cell culture into the calcium chloride solution using an air-atomizing device. When L. bulgaricus KFRI 673 was exposed to SGJ of pH 2.0 for 60 min, none of the microorganism survived. Contrary to this result, microbiological analysis indicated that microencapsulation in alginate microparticles improved the survival of acid-sensitive L. bulgaricus KFRI 673 in SGJ and that high molecular weight chitosan coating resulted in the highest survival in SGJ. To study storage stability of free and microencapsulated cells, in vitro studies were conducted at 4 and 22 °C during a 4 week period. Both free and microencapsulated cells showed similar stabilities during 4 weeks of storage at 4 °C. However, the stability of Lactobacillus at 22 °C was appreciably improved when loaded in high molecular weight chitosan-coated alginate microparticles. In conclusion, microencapsulation of lactic acid bacteria with alginate and chitosan coating offers an effective way of delivering viable bacterial cells to the colon and maintaining their survival during refrigerated storage.",
keywords = "Alginate, Chitosan coating, Lactobacillus bulgaricus, Microparticles, Storage stability",
author = "Lee, {J. S.} and Cha, {D. S.} and Park, {Hyun Jin}",
year = "2004",
month = "12",
day = "1",
doi = "10.1021/jf040235k",
language = "English",
volume = "52",
pages = "7300--7305",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "24",

}

TY - JOUR

T1 - Survival of freeze-dried Lactobacillus bulgaricus KFRI 673 in chitosan-coated calcium alginate microparticles

AU - Lee, J. S.

AU - Cha, D. S.

AU - Park, Hyun Jin

PY - 2004/12/1

Y1 - 2004/12/1

N2 - The aim of this study was to investigate the effect of alginate microparticles coated with three kinds of chitosans of different molecular weights on the survival of Lactobacillus bulgaricus KFRI 673 in simulated gastric (SGJ) and intestinal juices (SIJ) and on their stability during storage at 4 and 22 °C. L. bulgaricus KFRI 673 loaded in alginate microparticles was prepared by spraying the mixture of sodium alginate and cell culture into the calcium chloride solution using an air-atomizing device. When L. bulgaricus KFRI 673 was exposed to SGJ of pH 2.0 for 60 min, none of the microorganism survived. Contrary to this result, microbiological analysis indicated that microencapsulation in alginate microparticles improved the survival of acid-sensitive L. bulgaricus KFRI 673 in SGJ and that high molecular weight chitosan coating resulted in the highest survival in SGJ. To study storage stability of free and microencapsulated cells, in vitro studies were conducted at 4 and 22 °C during a 4 week period. Both free and microencapsulated cells showed similar stabilities during 4 weeks of storage at 4 °C. However, the stability of Lactobacillus at 22 °C was appreciably improved when loaded in high molecular weight chitosan-coated alginate microparticles. In conclusion, microencapsulation of lactic acid bacteria with alginate and chitosan coating offers an effective way of delivering viable bacterial cells to the colon and maintaining their survival during refrigerated storage.

AB - The aim of this study was to investigate the effect of alginate microparticles coated with three kinds of chitosans of different molecular weights on the survival of Lactobacillus bulgaricus KFRI 673 in simulated gastric (SGJ) and intestinal juices (SIJ) and on their stability during storage at 4 and 22 °C. L. bulgaricus KFRI 673 loaded in alginate microparticles was prepared by spraying the mixture of sodium alginate and cell culture into the calcium chloride solution using an air-atomizing device. When L. bulgaricus KFRI 673 was exposed to SGJ of pH 2.0 for 60 min, none of the microorganism survived. Contrary to this result, microbiological analysis indicated that microencapsulation in alginate microparticles improved the survival of acid-sensitive L. bulgaricus KFRI 673 in SGJ and that high molecular weight chitosan coating resulted in the highest survival in SGJ. To study storage stability of free and microencapsulated cells, in vitro studies were conducted at 4 and 22 °C during a 4 week period. Both free and microencapsulated cells showed similar stabilities during 4 weeks of storage at 4 °C. However, the stability of Lactobacillus at 22 °C was appreciably improved when loaded in high molecular weight chitosan-coated alginate microparticles. In conclusion, microencapsulation of lactic acid bacteria with alginate and chitosan coating offers an effective way of delivering viable bacterial cells to the colon and maintaining their survival during refrigerated storage.

KW - Alginate

KW - Chitosan coating

KW - Lactobacillus bulgaricus

KW - Microparticles

KW - Storage stability

UR - http://www.scopus.com/inward/record.url?scp=9344268283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9344268283&partnerID=8YFLogxK

U2 - 10.1021/jf040235k

DO - 10.1021/jf040235k

M3 - Article

VL - 52

SP - 7300

EP - 7305

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 24

ER -