Suspension culture of an antibacterial peptide producing cell line from Bombina orientalis

Yong Hwan Kim, Jae Won Yang, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The suspension culture of an anchorage-dependent cell line (Bok-1) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth (6.0 x 106 cells/ml) and viability (>80%) at 10 rpm were higher than that at 0 rpm (1.9 x 106 cells/ml, 65~80%) and 30 rpm (1.8 x 106 cells/ml, 40~76%). The size of cells became smaller at the agitation rate of 30 rpm. The antibacterial activities of cell extracts from suspension cultured cells were confirmed against gram-negative and gram-positive bacteria by the inhibition zone assay and the liquid growth inhibition assay.

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalJournal of microbiology and biotechnology
Volume8
Issue number5
Publication statusPublished - 1998

Keywords

  • Agitation rate
  • Antibacterial peptide
  • Bok-1
  • Shear stress
  • Suspension culture

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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