Abstract
The suspension culture of an anchorage-dependent cell line (Bok-1) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth (6.0 x 106 cells/ml) and viability (>80%) at 10 rpm were higher than that at 0 rpm (1.9 x 106 cells/ml, 65~80%) and 30 rpm (1.8 x 106 cells/ml, 40~76%). The size of cells became smaller at the agitation rate of 30 rpm. The antibacterial activities of cell extracts from suspension cultured cells were confirmed against gram-negative and gram-positive bacteria by the inhibition zone assay and the liquid growth inhibition assay.
Original language | English |
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Pages (from-to) | 461-465 |
Number of pages | 5 |
Journal | Journal of microbiology and biotechnology |
Volume | 8 |
Issue number | 5 |
Publication status | Published - 1998 |
Keywords
- Agitation rate
- Antibacterial peptide
- Bok-1
- Shear stress
- Suspension culture
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology