Synaptic transmission in human neocortex removed for treatment of intractable epilepsy in children

Jean Pierre Wuarin, Yang In Kim, Carlos Cepeda, Jeffrey G. Tasker, John P. Walsh, Warwick J. Peacock, Nathaniel A. Buchwald, F. Edward Dudek

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Synaptic transmission to pyramidal cells was studied in slices of neocortex resected from infants and children (n = 10, age 8 months to 13 years) undergoing surgical treatment for intractable epilepsy. Most specimens were from the least abnormal area of the resection. Stable intracellular recordings could be obtained for up to 8 hours. Most of the recorded neurons had electrophysiological characteristics similar to those of regular-firing pyramidal cells and were in layers III to V, which was confirmed by intracellular staining with Lucifer yellow. Local extracellular stimulation evoked a sequence of excitatory and inhibitory postsynaptic potentials. Kynurenic acid, a broad-spectrum excitatory amino acid antagonist, depressed the evoked excitatory postsynaptic potentials. After application of the gamma-aminobutyric acid antagonist, bicuculline (10-30 μM), extracellular stimulation induced large excitatory postsynaptic potentials and epileptiform bursts. Spontaneous bursts occasionally occurred in bicuculline. This effect of bicuculline was observed in all the tissue samples, even those from infant patients (n = 4, age 8-16 months). Kynurenic acid depressed or abolished both spontaneous and stimulation-induced bursts. The competitive antagonist for N-methyl-D-aspartate receptors, DL-2-amino-5-phosphonopentanoic acid decreased the duration of bicuculline-induced bursts. These data provide evidence that, similar to rat and cat neocortex, excitatory and inhibitory amino acids are important transmitters to pyramidal cells in immature human neocortex.

Original languageEnglish
Pages (from-to)503-511
Number of pages9
JournalAnnals of Neurology
Volume28
Issue number4
Publication statusPublished - 1990 Oct 1
Externally publishedYes

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Bicuculline
Neocortex
Synaptic Transmission
Pyramidal Cells
Excitatory Postsynaptic Potentials
Kynurenic Acid
GABA Antagonists
2-Amino-5-phosphonovalerate
Excitatory Amino Acid Antagonists
Inhibitory Postsynaptic Potentials
Excitatory Amino Acids
Therapeutics
N-Methyl-D-Aspartate Receptors
Cats
Staining and Labeling
Neurons
Drug Resistant Epilepsy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wuarin, J. P., Kim, Y. I., Cepeda, C., Tasker, J. G., Walsh, J. P., Peacock, W. J., ... Dudek, F. E. (1990). Synaptic transmission in human neocortex removed for treatment of intractable epilepsy in children. Annals of Neurology, 28(4), 503-511.

Synaptic transmission in human neocortex removed for treatment of intractable epilepsy in children. / Wuarin, Jean Pierre; Kim, Yang In; Cepeda, Carlos; Tasker, Jeffrey G.; Walsh, John P.; Peacock, Warwick J.; Buchwald, Nathaniel A.; Dudek, F. Edward.

In: Annals of Neurology, Vol. 28, No. 4, 01.10.1990, p. 503-511.

Research output: Contribution to journalArticle

Wuarin, JP, Kim, YI, Cepeda, C, Tasker, JG, Walsh, JP, Peacock, WJ, Buchwald, NA & Dudek, FE 1990, 'Synaptic transmission in human neocortex removed for treatment of intractable epilepsy in children', Annals of Neurology, vol. 28, no. 4, pp. 503-511.
Wuarin JP, Kim YI, Cepeda C, Tasker JG, Walsh JP, Peacock WJ et al. Synaptic transmission in human neocortex removed for treatment of intractable epilepsy in children. Annals of Neurology. 1990 Oct 1;28(4):503-511.
Wuarin, Jean Pierre ; Kim, Yang In ; Cepeda, Carlos ; Tasker, Jeffrey G. ; Walsh, John P. ; Peacock, Warwick J. ; Buchwald, Nathaniel A. ; Dudek, F. Edward. / Synaptic transmission in human neocortex removed for treatment of intractable epilepsy in children. In: Annals of Neurology. 1990 ; Vol. 28, No. 4. pp. 503-511.
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