Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS)

Ji Hoon Koh, Seung Hye Choi, Seung Won Park, Nag Jin Choi, Younghoon Kim, Sae Hun Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Synbiotics, the combination of prebiotics and probiotics, has been shown to produce synergistic effects that promote gastrointestinal well-being of host. Tagatose is a low calorie food ingredient with putative health-promoting benefits. Herein, we investigated its synbiotic impact on the viability of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and the potential mechanism involved. Tagatose, as a synbiotic substrate, enhanced the growth of L.casei 01 and L.rhamnosus strain GG compared to other prebiotics. Other gut-indigenous such as Clostridium spp. readily utilized fructooligosaccharide (FOS), the most widely used functional prebiotics, but not tagatose. Additionally, tagatose enhanced probiotic functions of L.casei 01 and L.rhamnosus strain GG by reinforcing their attachment on HT-29 intestine epithelial cells and enhancing their cholesterol-lowering activities. Whole transcriptome study and quantitative real-time polymerase chain reaction (qRT-PCR) test showed that the presence of tagatose in L.rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system (PTS). Collectively, these results indicate the tagatose enhanced the growth of L.casei 01 and L.rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks. Importantly, this study highlights the potential application of tagatose and L.casei 01 and/or L.rhamnosus strain GG as a synbiotic partner in functional dairy foods (i.e. yogurt and cheese) and therapeutic dietary supplements.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalFood Microbiology
Volume36
Issue number1
DOIs
Publication statusPublished - 2013 Oct 1

Fingerprint

Synbiotics
Lactobacillus rhamnosus
dietary supplements
phosphotransferases (kinases)
Phosphotransferases
viability
Prebiotics
Probiotics
prebiotics
probiotics
low calorie foods
Lactobacillus casei
Yogurt
health promotion
Functional Food
fructooligosaccharides
Clostridium
tagatose
Carbohydrate Metabolism
Cheese

Keywords

  • DNA microarray
  • Probiotic bacteria
  • Synbiotics
  • Tagatose

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS). / Koh, Ji Hoon; Choi, Seung Hye; Park, Seung Won; Choi, Nag Jin; Kim, Younghoon; Kim, Sae Hun.

In: Food Microbiology, Vol. 36, No. 1, 01.10.2013, p. 7-13.

Research output: Contribution to journalArticle

Koh, Ji Hoon ; Choi, Seung Hye ; Park, Seung Won ; Choi, Nag Jin ; Kim, Younghoon ; Kim, Sae Hun. / Synbiotic impact of tagatose on viability of Lactobacillus rhamnosus strain GG mediated by the phosphotransferase system (PTS). In: Food Microbiology. 2013 ; Vol. 36, No. 1. pp. 7-13.
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