TY - JOUR
T1 - Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose
AU - Kim, In Jung
AU - Youn, Hak Jin
AU - Kim, Kyoung Heon
N1 - Funding Information:
We want to acknowledge grant support from the Ministry of Trade, Industry & Energy ( 10049674 ) and facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. IJK is grateful for a grant from Korea University .
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.
AB - Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.
KW - Auxiliary activity 9
KW - Chaetomium globosum
KW - Lytic polysaccharide monooxygenase
KW - Synergism
KW - Xylan
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U2 - 10.1016/j.procbio.2016.06.017
DO - 10.1016/j.procbio.2016.06.017
M3 - Article
AN - SCOPUS:84979697119
VL - 51
SP - 1445
EP - 1451
JO - Process Biochemistry
JF - Process Biochemistry
SN - 1359-5113
IS - 10
ER -