Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose

In Jung Kim, Hak Jin Youn, Kyoung Heon Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. . Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from . Chaetomium globosum (CgAA9) was identified and expressed in . Escherichia coli. The recombinant . CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that . CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, . CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by . CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, . CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.

Original languageEnglish
JournalProcess Biochemistry
DOIs
Publication statusAccepted/In press - 2016 Apr 19

Fingerprint

Chaetomium
Xylans
Hydrolysis
Cellulose
Fungal Proteins
Cellulase
Ascorbic acid
Straw
Polysaccharides
Mixed Function Oxygenases
Sugars
Escherichia coli
Ascorbic Acid
lignocellulose

Keywords

  • Auxiliary activity 9
  • Chaetomium globosum
  • Lytic polysaccharide monooxygenase
  • Synergism
  • Xylan

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

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title = "Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose",
abstract = "Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. . Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from . Chaetomium globosum (CgAA9) was identified and expressed in . Escherichia coli. The recombinant . CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that . CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, . CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by . CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, . CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.",
keywords = "Auxiliary activity 9, Chaetomium globosum, Lytic polysaccharide monooxygenase, Synergism, Xylan",
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AU - Kim, In Jung

AU - Youn, Hak Jin

AU - Kim, Kyoung Heon

PY - 2016/4/19

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N2 - Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. . Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from . Chaetomium globosum (CgAA9) was identified and expressed in . Escherichia coli. The recombinant . CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that . CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, . CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by . CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, . CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.

AB - Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. . Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from . Chaetomium globosum (CgAA9) was identified and expressed in . Escherichia coli. The recombinant . CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that . CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, . CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by . CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, . CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.

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KW - Synergism

KW - Xylan

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