Synergistic activities of gaseous oregano and thyme thymol essential oils against Listeria monocytogenes on surfaces of a laboratory medium and radish sprouts

Yurim Cho, Hoikyung Kim, Larry R. Beuchat, Jee Hoon Ryu

Research output: Contribution to journalArticle

Abstract

We investigated combinations of gaseous essential oils (EO gases) for their synergistic inhibitory activities against Listeria monocytogenes on a laboratory medium and radish sprouts. The minimum inhibitory concentrations and minimum lethal concentrations of oregano, thyme thymol, and cinnamon bark EO gases against L. monocytogenes were 0.0781 μL/mL on nutrient agar supplemented with glucose and bromocresol purple (NGBA). A checkerboard assay showed that combinations of oregano and thyme thymol EO gases and of oregano and cinnamon bark EO gases exert the strongest synergistic antilisterial activity (fractional inhibitory concentration index [FICI] = 0.3750). A combination of thyme thymol and cinnamon bark EO gases also had a synergistic effect (FICI = 0.5000) on L. monocytogenes on NGBA. Combinations of oregano and thyme thymol EO gases were tested for synergistic antimicrobial activity against L. monocytogenes on radish sprouts. A combination of these gases, each at 0.313 μL/mL, caused a significant (P ≤ 0.05) reduction in the number of L. monocytogenes on radish sprouts compared with reductions caused by treatment with oregano or thyme thymol EO gas alone at the same concentration. Our findings provide information that will be useful when developing antimicrobial applications using EO gases to control L. monocytogenes in the food industry.

Original languageEnglish
Article number103357
JournalFood Microbiology
Volume86
DOIs
Publication statusPublished - 2020 Apr

Fingerprint

Thymus Plant
Origanum
Thymol
Raphanus
sprouts (food)
oregano
thyme
thymol
Listeria monocytogenes
Volatile Oils
radishes
essential oils
Gases
gases
Cinnamomum zeylanicum
cinnamon
bark
Bromcresol Purple
anti-infective agents
Food Industry

Keywords

  • Fresh produce
  • Gaseous essential oil
  • Listeria monocytogenes
  • Radish sprouts
  • Synergistic antilisterial activity

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Synergistic activities of gaseous oregano and thyme thymol essential oils against Listeria monocytogenes on surfaces of a laboratory medium and radish sprouts. / Cho, Yurim; Kim, Hoikyung; Beuchat, Larry R.; Ryu, Jee Hoon.

In: Food Microbiology, Vol. 86, 103357, 04.2020.

Research output: Contribution to journalArticle

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