Synergistic antimicrobial activity of metabolites produced by a nonobligate bacterial predator

Cody C. Cain, Dongho Lee, Robert H. Waldo, Alexis T. Henry, Earl J. Casida, Mansukh C. Wani, Monroe E. Wall, Nicholas H. Oberlies, Joseph O. Falkinham

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A naturally occurring, gram-negative, nonobligate predator bacterial strain 679-2, exhibits broad-spectrum antimicrobial activity that is due, in part, to the production of three extracellular compounds. Antimicrobial-activity-directed fractionation of a culture of strain 679-2 against a panel of microorganisms has led to the isolation of three compounds: pyrrolnitrin, maculosin, and a new compound, which we have named banegasine. Although pyrroinitrin is well known in the literature, it has not been found in cells with the herbicide maculosin. Further, this is the first report of production of maculosin by a prokaryote. Both maculosin and banegasine, which displayed no antimicrobial activities alone, were found to potentiate the antimicrobial activity of pyrroinitrin. Based on 16S rRNA sequence, cellular fatty acid composition, and biochemical and cultural characteristics, strain 679-2 appears to represent a new genus and species of eubacteria, Aristabacter necator. The potent, broad-spectrum antimicrobial activity of predator strain 679-2 may be due to synergism between metabolites.

Original languageEnglish
Pages (from-to)2113-2117
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume47
Issue number7
DOIs
Publication statusPublished - 2003 Jul 1
Externally publishedYes

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Pyrrolnitrin
Necator
Cultural Characteristics
Herbicides
Fatty Acids
Bacteria
maculosin

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Synergistic antimicrobial activity of metabolites produced by a nonobligate bacterial predator. / Cain, Cody C.; Lee, Dongho; Waldo, Robert H.; Henry, Alexis T.; Casida, Earl J.; Wani, Mansukh C.; Wall, Monroe E.; Oberlies, Nicholas H.; Falkinham, Joseph O.

In: Antimicrobial Agents and Chemotherapy, Vol. 47, No. 7, 01.07.2003, p. 2113-2117.

Research output: Contribution to journalArticle

Cain, CC, Lee, D, Waldo, RH, Henry, AT, Casida, EJ, Wani, MC, Wall, ME, Oberlies, NH & Falkinham, JO 2003, 'Synergistic antimicrobial activity of metabolites produced by a nonobligate bacterial predator', Antimicrobial Agents and Chemotherapy, vol. 47, no. 7, pp. 2113-2117. https://doi.org/10.1128/AAC.47.7.2113-2117.2003
Cain, Cody C. ; Lee, Dongho ; Waldo, Robert H. ; Henry, Alexis T. ; Casida, Earl J. ; Wani, Mansukh C. ; Wall, Monroe E. ; Oberlies, Nicholas H. ; Falkinham, Joseph O. / Synergistic antimicrobial activity of metabolites produced by a nonobligate bacterial predator. In: Antimicrobial Agents and Chemotherapy. 2003 ; Vol. 47, No. 7. pp. 2113-2117.
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