Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase

Rui Nian, Duck Sang Kim, Lihan Tan, Chan Wha Kim, Woo Seok Choe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperonemediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large-scale refolding processes.

Original languageEnglish
Pages (from-to)1078-1085
Number of pages8
JournalBiotechnology Progress
Volume25
Issue number4
DOIs
Publication statusPublished - 2009 Jul 1

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Keywords

  • Malate dehydrogenase (MDH)
  • Molecular bichaperone
  • Polyethylene glycol (PEG)
  • Protein refolding

ASJC Scopus subject areas

  • Biotechnology

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