Synergistic effect of chlorine dioxide and drying treatments for nactivatmg Escherichia coli 0157: H7 on radish seeds

Hoik Yung Kim, Haeyoung Kim, Jihyun Bang, Larry R. Beuchat, Jee-Hoon Ryu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Studies were done to determine whether calcium hypochlorite (Ca(OCl) 2) and chlorine dioxide (C1O2) treatment followed by drying had a synergistic killing effect on microorganisms on radish seeds intended for sprout production. Uninoculated radish seeds and seeds inoculated with Escherichia coli O157: H7 were treated with water, Ca(OCl)2 (free chlorine concentrations of 50 or 200 μg/ml), or C1O2 (50 or 200 μg/ml) for 5 min and subsequently dried at 25°C for up to 24 h. Populations of total aerobic bacteria (TAB), molds and yeasts (MY), and E. coli O157: H7 on the seeds treated with Ca(OCl)2 were not significantly different (P = 0.05) than populations on seeds treated with C1O2 at the same concentrations. However, populations of microorganisms on seeds treated with C1O2 decreased more rapidly during drying. Treatment with C1O2 (200 μg/ml) followed by drying caused reductions in TAB, MY, and E. coli 0157: H7 of 3.1, 2.0, and 3.8 log CFU/g, respectively. When seeds were treated with water, Ca(OCl)2 (50 or 200 μg/ml), and C1O 2 (50 μg/ml) and subsequently dried, reductions in TAB, MY, and E. coli O157: H7 were 0.2 to 2.0, 0.4 to 2.0, and 1.4 to 2.2 log CFU/g, respectively. Results indicate that inactivation of E. coli OL57: H7 on radish seeds is greater after treatment with C1O2 followed by drying than after treatment with Ca(OCl)2 followed by drying, thus providing a synergistic treatment combination for reducing the safety risk associated with sprouts produced from these seeds.

Original languageEnglish
Pages (from-to)1225-1230
Number of pages6
JournalJournal of Food Protection
Volume73
Issue number7
Publication statusPublished - 2010 Jul 1

Fingerprint

chlorine dioxide
Raphanus
radishes
Seeds
drying
Escherichia coli
seeds
Aerobic Bacteria
Escherichia coli O157
molds (fungi)
Fungi
Yeasts
yeasts
bacteria
calcium hypochlorite
Population
microorganisms
Water
Chlorine
chlorine

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Synergistic effect of chlorine dioxide and drying treatments for nactivatmg Escherichia coli 0157 : H7 on radish seeds. / Kim, Hoik Yung; Kim, Haeyoung; Bang, Jihyun; Beuchat, Larry R.; Ryu, Jee-Hoon.

In: Journal of Food Protection, Vol. 73, No. 7, 01.07.2010, p. 1225-1230.

Research output: Contribution to journalArticle

Kim, Hoik Yung ; Kim, Haeyoung ; Bang, Jihyun ; Beuchat, Larry R. ; Ryu, Jee-Hoon. / Synergistic effect of chlorine dioxide and drying treatments for nactivatmg Escherichia coli 0157 : H7 on radish seeds. In: Journal of Food Protection. 2010 ; Vol. 73, No. 7. pp. 1225-1230.
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