Synergistic Effect of Detection and Separation for Pathogen Using Magnetic Clusters

Yong Tae Kim, Kook Han Kim, Eun Sung Kang, Geoncheol Jo, Se Young Ahn, Seon Hwa Park, Sung Il Kim, Saem Mun, Kyuwon Baek, Byeongyoon Kim, Kwangyeol Lee, Wan Soo Yun, Yong Ho Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Early diagnosis of infectious diseases is important for treatment; therefore, selective and rapid detection of pathogenic bacteria is essential for human health. We report a strategy for highly selective detection and rapid separation of pathogenic microorganisms using magnetic nanoparticle clusters. Our approach to develop probes for pathogenic bacteria, including Salmonella, is based on a theoretically optimized model for the size of clustered magnetic nanoparticles. The clusters were modified to provide enhanced aqueous solubility and versatile conjugation sites for antibody immobilization. The clusters with the desired magnetic property were then prepared at critical micelle concentration (CMC) by evaporation-induced self-assembly (EISA). Two different types of target-specific antibodies for H- and O-antigens were incorporated on the cluster surface for selective binding to biological compartments of the flagella and cell body, respectively. For the two different specific binding properties, Salmonella were effectively captured with the O-antibody-coated polysorbate 80-coated magnetic nanoclusters (PCMNCs). The synergistic effect of combining selective targeting and the clustered magnetic probe leads to both selective and rapid detection of infectious pathogens.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalBioconjugate Chemistry
Volume27
Issue number1
DOIs
Publication statusPublished - 2016 Jan 20

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Kim, Y. T., Kim, K. H., Kang, E. S., Jo, G., Ahn, S. Y., Park, S. H., Kim, S. I., Mun, S., Baek, K., Kim, B., Lee, K., Yun, W. S., & Kim, Y. H. (2016). Synergistic Effect of Detection and Separation for Pathogen Using Magnetic Clusters. Bioconjugate Chemistry, 27(1), 59-65. https://doi.org/10.1021/acs.bioconjchem.5b00681