Synergistic effect of the enzyme complexes comprising agarase, carrageenase and neoagarobiose hydrolase on degradation of the red algae

Dae Hee Kang, Seung Kyou You, Young Chul Joo, Sang Kyu Shin, Jeong Eun Hyeon, Sung Ok Han

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the practice of converting red algae biomass into biofuel or valuable biomaterials, the critical step is the decomposition process of the agarose to give fermentable monomeric sugars. In this study, we selected three enzymes such as agarase, carrageenase and neoagarobiose hydrolase to inducible the simultaneous hydrolysis of the major substrates such as agar and carrageenan constituting the pretreated red algae, and expressed the chimeric enzymes and formed a complexes through optimization of addition ratio. As a result, hydrolysis by enzyme complexes showed a maximum sugar release of 679 mg L−1 with 67.9% saccharification yield from G. verrucosa natural substrate. The difference in the reducing sugar by the enzyme complexes was 3.6-fold higher than that of the monomer enzyme (cAgaB yield 188.6 mg L−1). The synergistic effect of producing sugars from red algae biomass through these enzyme complexes can be a very important biological tools aimed at bioenergy production.

Original languageEnglish
Pages (from-to)666-672
Number of pages7
JournalBioresource Technology
Volume250
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Hydrolases
red alga
Algae
Enzymes
enzyme
Sugars
Degradation
degradation
sugar
hydrolysis
Hydrolysis
Biomass
carrageenan
substrate
Saccharification
Biofuels
Carrageenan
biomass
bioenergy
Biocompatible Materials

Keywords

  • Agarase
  • Carrageenase
  • Gracilaria verrucosa
  • Hydrolytic enzyme complexes
  • Neoagarobiose hydrolase

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Synergistic effect of the enzyme complexes comprising agarase, carrageenase and neoagarobiose hydrolase on degradation of the red algae. / Kang, Dae Hee; You, Seung Kyou; Joo, Young Chul; Shin, Sang Kyu; Hyeon, Jeong Eun; Han, Sung Ok.

In: Bioresource Technology, Vol. 250, 01.02.2018, p. 666-672.

Research output: Contribution to journalArticle

Kang, Dae Hee ; You, Seung Kyou ; Joo, Young Chul ; Shin, Sang Kyu ; Hyeon, Jeong Eun ; Han, Sung Ok. / Synergistic effect of the enzyme complexes comprising agarase, carrageenase and neoagarobiose hydrolase on degradation of the red algae. In: Bioresource Technology. 2018 ; Vol. 250. pp. 666-672.
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