Synergistic stimulation of surface topography and biphasic electric current promotes muscle regeneration

Indong Jun, Na Li, Jaehee Shin, Jaeho Park, Young Jun Kim, Hojeong Jeon, Hyuk Choi, Jae Gu Cho, Byoung Chan Choi, Hyung Seop Han, Jae Jun Song

Research output: Contribution to journalArticlepeer-review

Abstract

Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment. This study focuses on the application of topographical and electrical field stimuli to human myogenic precursor cell (hMPC) cultures to assess the influences of the adherent direction, proliferation, and differentiation, and induce preconditioning-induced therapeutic benefits. First, a topographical surface of commercially available culture dishes was achieved by femtosecond laser texturing. The detachable biphasic electrical current system was then applied to the hMPCs cultured on laser-textured culture dishes. Laser-textured topographies were remarkably effective in inducing the assembly of hMPC myotubes by enhancing the orientation of adherent hMPCs compared with flat surfaces. Furthermore, electrical field stimulation through laser-textured topographies was found to promote the expression of myogenic regulatory factors compared with nonstimulated cells. As such, we successfully demonstrated that the combined stimulation of topographical and electrical cues could effectively enhance the myogenic maturation of hMPCs in a surface spatial and electrical field-dependent manner, thus providing the basis for therapeutic strategies.

Original languageEnglish
Pages (from-to)118-129
Number of pages12
JournalBioactive Materials
Volume11
DOIs
Publication statusPublished - 2022 May

Keywords

  • Cell therapy
  • Electrical field stimulation
  • Femtosecond laser
  • Human myogenic precursor cell
  • Topography

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biomedical Engineering

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