Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

Myung Ik Yoo, Yeong Ju Seo, Kyu Sil Choi, Jeong Sook Ha, Kyoungja Woo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume97
DOIs
Publication statusPublished - 2012 Sep 1

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Iron oxides
iron oxides
Integrins
Nanoparticles
nanoparticles
Acids
Water
MCF-7 Cells
synthesis
cells
Endothelial cells
acids
Oleic Acid
Magnetic resonance imaging
water
Ligands
Cells
cultured cells
Endothelial Cells
breast

Keywords

  • Cellular uptake
  • Lipophilic
  • Scatteredly cRGDfK-conjugated
  • Superparamagnetic iron oxide nanoparticles (SPIONs)
  • Ultrasmall
  • Water-dispersible

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles. / Yoo, Myung Ik; Seo, Yeong Ju; Choi, Kyu Sil; Ha, Jeong Sook; Woo, Kyoungja.

In: Colloids and Surfaces B: Biointerfaces, Vol. 97, 01.09.2012, p. 175-181.

Research output: Contribution to journalArticle

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