Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead

Hee Uk Lee, Yoon Seok Song, Young Joon Suh, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The goal of this study was to improve the activity and stability of glucose oxidase (GOD) immobilized on Co-B/SiO 2 nanoparticles (NPs) entrapped in chitosan beads. The Co-B/SiO 2 NPs were prepared from a silica shell-coated Co-B core using the Stöber method. GOD was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The optimal conditions for immobilization of GOD onto chitosan bead (IGCB) were a 1.2 wt% of chitosan solution, and 2.0 wt% of sodium tripolyphosphate (TPP). K M and V max were determined to be 60.7 mM and 43.5 μM/min for free GODs (FG), 15.2 mM and 4.8 μM/min for immobilized GODs (IG), and 51.2 mM and 5.0 μM/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IG and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalJournal of Molecular Catalysis B: Enzymatic
Volume81
DOIs
Publication statusPublished - 2012 Sep 1

Keywords

  • Chitosan bead
  • Co-B/SiO
  • Core/shell
  • Glucose oxidase
  • Immobilization

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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