Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead

Hee Uk Lee; Yoon Seok Song; Young Joon Suh; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.molcatb.2012.05.004

Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead

Hee Uk Lee, Yoon Seok Song, Young Joon Suh, Chulhwan Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The goal of this study was to improve the activity and stability of glucose oxidase (GOD) immobilized on Co-B/SiO ₂ nanoparticles (NPs) entrapped in chitosan beads. The Co-B/SiO ₂ NPs were prepared from a silica shell-coated Co-B core using the Stöber method. GOD was covalently immobilized on the surface of Co-B/SiO ₂/NH ₂ NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The optimal conditions for immobilization of GOD onto chitosan bead (IGCB) were a 1.2 wt% of chitosan solution, and 2.0 wt% of sodium tripolyphosphate (TPP). K _M and V _max were determined to be 60.7 mM and 43.5 μM/min for free GODs (FG), 15.2 mM and 4.8 μM/min for immobilized GODs (IG), and 51.2 mM and 5.0 μM/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IG and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively.

Original language	English
Pages (from-to)	31-36
Number of pages	6
Journal	Journal of Molecular Catalysis B: Enzymatic
Volume	81
DOIs	https://doi.org/10.1016/j.molcatb.2012.05.004
Publication status	Published - 2012 Sept

Keywords

Chitosan bead
Co-B/SiO
Core/shell
Glucose oxidase
Immobilization

ASJC Scopus subject areas

Catalysis
Bioengineering
Biochemistry
Process Chemistry and Technology

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10.1016/j.molcatb.2012.05.004

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title = "Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead",

abstract = "The goal of this study was to improve the activity and stability of glucose oxidase (GOD) immobilized on Co-B/SiO 2 nanoparticles (NPs) entrapped in chitosan beads. The Co-B/SiO 2 NPs were prepared from a silica shell-coated Co-B core using the St{\"o}ber method. GOD was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The optimal conditions for immobilization of GOD onto chitosan bead (IGCB) were a 1.2 wt% of chitosan solution, and 2.0 wt% of sodium tripolyphosphate (TPP). K M and V max were determined to be 60.7 mM and 43.5 μM/min for free GODs (FG), 15.2 mM and 4.8 μM/min for immobilized GODs (IG), and 51.2 mM and 5.0 μM/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IG and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively.",

keywords = "Chitosan bead, Co-B/SiO, Core/shell, Glucose oxidase, Immobilization",

author = "Lee, {Hee Uk} and Song, {Yoon Seok} and Suh, {Young Joon} and Chulhwan Park and Kim, {Seung Wook}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center ( ABC-2010-0029799 ) of Korea Grant funded by the Ministry of Education, Science and Technology .",

year = "2012",

month = sep,

doi = "10.1016/j.molcatb.2012.05.004",

language = "English",

volume = "81",

pages = "31--36",

journal = "Journal of Molecular Catalysis B: Enzymatic",

issn = "1381-1177",

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TY - JOUR

T1 - Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead

AU - Lee, Hee Uk

AU - Song, Yoon Seok

AU - Suh, Young Joon

AU - Park, Chulhwan

AU - Kim, Seung Wook

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center ( ABC-2010-0029799 ) of Korea Grant funded by the Ministry of Education, Science and Technology .

PY - 2012/9

Y1 - 2012/9

N2 - The goal of this study was to improve the activity and stability of glucose oxidase (GOD) immobilized on Co-B/SiO 2 nanoparticles (NPs) entrapped in chitosan beads. The Co-B/SiO 2 NPs were prepared from a silica shell-coated Co-B core using the Stöber method. GOD was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The optimal conditions for immobilization of GOD onto chitosan bead (IGCB) were a 1.2 wt% of chitosan solution, and 2.0 wt% of sodium tripolyphosphate (TPP). K M and V max were determined to be 60.7 mM and 43.5 μM/min for free GODs (FG), 15.2 mM and 4.8 μM/min for immobilized GODs (IG), and 51.2 mM and 5.0 μM/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IG and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively.

AB - The goal of this study was to improve the activity and stability of glucose oxidase (GOD) immobilized on Co-B/SiO 2 nanoparticles (NPs) entrapped in chitosan beads. The Co-B/SiO 2 NPs were prepared from a silica shell-coated Co-B core using the Stöber method. GOD was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The optimal conditions for immobilization of GOD onto chitosan bead (IGCB) were a 1.2 wt% of chitosan solution, and 2.0 wt% of sodium tripolyphosphate (TPP). K M and V max were determined to be 60.7 mM and 43.5 μM/min for free GODs (FG), 15.2 mM and 4.8 μM/min for immobilized GODs (IG), and 51.2 mM and 5.0 μM/min for IGCB, respectively. After being entrapped into the chitosan beads, the GOD exhibited improved storage and operation stability. The IG and IGCB retained 48% and 64% of their initial activity after 7 reuses, respectively.

KW - Chitosan bead

KW - Co-B/SiO

KW - Core/shell

KW - Glucose oxidase

KW - Immobilization

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DO - 10.1016/j.molcatb.2012.05.004

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AN - SCOPUS:84861669159

SN - 1381-1177

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SP - 31

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JO - Journal of Molecular Catalysis B: Enzymatic

JF - Journal of Molecular Catalysis B: Enzymatic

ER -

Synthesis and characterization of glucose oxidase-core/shell magnetic nanoparticle complexes into chitosan bead

Abstract

Keywords

ASJC Scopus subject areas

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