Synthesis and characterization of pullulan-mediated silver nanoparticles and its antimicrobial activities

Paulraj Kanmani, Seung Taik Lim

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Synthesis of silver nanoparticles was achieved using pullulan as both a reducing and stabilizing agent. The effect of pullulan and silver nitrate amounts on the synthesis of silver nanoparticles (AgNPs) was investigated. The formation of nanoparticles was first screened by measuring the surface plasmon resonance peak at 420-430 nm using UV-vis spectroscopy. The morphology of the synthesized AgNPs was determined using TEM, which indicated that the AgNPs varied in shape and polydispersed with an average size of 2-30 nm. The presence of elemental silver and the crystalline structure of the AgNPs were confirmed by EDX and XRD analyses. The possible functional groups of pullulan responsible for the reduction and stabilization of AgNPs were evaluated using FT-IR. The pullulan-reduced AgNPs showed excellent antibacterial, antifungal, and antibiofilm activity against food and multidrug resistant bacterial and fungal pathogens. The results showed that pullulan could be used as a reducing as well as a capping agent for synthesizing AgNPs which had potent antimicrobial activity.

Original languageEnglish
Pages (from-to)421-428
Number of pages8
JournalCarbohydrate Polymers
Volume97
Issue number2
DOIs
Publication statusPublished - 2013 Oct 17

Fingerprint

Silver
Nanoparticles
Surface plasmon resonance
Pathogens
Silver Nitrate
Ultraviolet spectroscopy
Functional groups
Excipients
Reducing Agents
Energy dispersive spectroscopy
Nitrates
Stabilization
Crystalline materials
Transmission electron microscopy
pullulan

Keywords

  • Antibacterial
  • Antibiofilm
  • Antifungal
  • Pullulan
  • Silver nanoparticles

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Synthesis and characterization of pullulan-mediated silver nanoparticles and its antimicrobial activities. / Kanmani, Paulraj; Lim, Seung Taik.

In: Carbohydrate Polymers, Vol. 97, No. 2, 17.10.2013, p. 421-428.

Research output: Contribution to journalArticle

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