Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase

Heejin Kim, Nakyung Choi, Se Wook Oh, Yangha Kim, Byung Hee Kim, In-Hwan Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An α-linolenic acid (ALA)-rich triacylglycerol (TAG) was synthesized from an ALA-rich fatty acid (FA) from perilla oil and glycerol, using a newly prepared immobilized lipase under vacuum. The ALA-rich FA (purity >90 wt%) used as the substrate was prepared by urea complexation from perilla oil FAs. Liquid Lipozyme TL 100 L lipase from Thermomyces lanuginosus was used for immobilization. Nine different hydrophilic and hydrophobic carriers for immobilization were tested, and Duolite A568, which is a hydrophilic resin, was selected as the best carrier. This immobilized lipase was used to synthesize TAG by direct esterification under vacuum. The parameters investigated were temperature, enzyme loading, and vacuum level. The optimum reaction conditions were a temperature of 60 °C, an enzyme loading of 15% (based on the total weight of the substrate), and a vacuum of 0.7 kPa, respectively. The maximum conversion to TAG of ca. 88 wt% was obtained in 12 h under the optimum conditions.

Original languageEnglish
Pages (from-to)654-658
Number of pages5
JournalFood Chemistry
Volume237
DOIs
Publication statusPublished - 2017 Dec 15

Fingerprint

alpha-Linolenic Acid
Vacuum
linolenic acid
Lipase
Triglycerides
triacylglycerols
synthesis
Thermomyces lanuginosus
Immobilization
fatty acids
Fatty Acids
esterification
enzymes
purity
Temperature
resins
glycerol
Esterification
temperature
Substrates

Keywords

  • Duolite A568
  • Immobilization
  • Lipase
  • Thermomyces lanuginosus
  • Triacylglycerol
  • α-Linolenic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase. / Kim, Heejin; Choi, Nakyung; Oh, Se Wook; Kim, Yangha; Hee Kim, Byung; Kim, In-Hwan.

In: Food Chemistry, Vol. 237, 15.12.2017, p. 654-658.

Research output: Contribution to journalArticle

Kim, Heejin ; Choi, Nakyung ; Oh, Se Wook ; Kim, Yangha ; Hee Kim, Byung ; Kim, In-Hwan. / Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase. In: Food Chemistry. 2017 ; Vol. 237. pp. 654-658.
@article{c029dd83709f4f92ae17a9867dfd0818,
title = "Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase",
abstract = "An α-linolenic acid (ALA)-rich triacylglycerol (TAG) was synthesized from an ALA-rich fatty acid (FA) from perilla oil and glycerol, using a newly prepared immobilized lipase under vacuum. The ALA-rich FA (purity >90 wt{\%}) used as the substrate was prepared by urea complexation from perilla oil FAs. Liquid Lipozyme TL 100 L lipase from Thermomyces lanuginosus was used for immobilization. Nine different hydrophilic and hydrophobic carriers for immobilization were tested, and Duolite A568, which is a hydrophilic resin, was selected as the best carrier. This immobilized lipase was used to synthesize TAG by direct esterification under vacuum. The parameters investigated were temperature, enzyme loading, and vacuum level. The optimum reaction conditions were a temperature of 60 °C, an enzyme loading of 15{\%} (based on the total weight of the substrate), and a vacuum of 0.7 kPa, respectively. The maximum conversion to TAG of ca. 88 wt{\%} was obtained in 12 h under the optimum conditions.",
keywords = "Duolite A568, Immobilization, Lipase, Thermomyces lanuginosus, Triacylglycerol, α-Linolenic acid",
author = "Heejin Kim and Nakyung Choi and Oh, {Se Wook} and Yangha Kim and {Hee Kim}, Byung and In-Hwan Kim",
year = "2017",
month = "12",
day = "15",
doi = "10.1016/j.foodchem.2017.05.161",
language = "English",
volume = "237",
pages = "654--658",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Synthesis of α-linolenic acid-rich triacylglycerol using a newly prepared immobilized lipase

AU - Kim, Heejin

AU - Choi, Nakyung

AU - Oh, Se Wook

AU - Kim, Yangha

AU - Hee Kim, Byung

AU - Kim, In-Hwan

PY - 2017/12/15

Y1 - 2017/12/15

N2 - An α-linolenic acid (ALA)-rich triacylglycerol (TAG) was synthesized from an ALA-rich fatty acid (FA) from perilla oil and glycerol, using a newly prepared immobilized lipase under vacuum. The ALA-rich FA (purity >90 wt%) used as the substrate was prepared by urea complexation from perilla oil FAs. Liquid Lipozyme TL 100 L lipase from Thermomyces lanuginosus was used for immobilization. Nine different hydrophilic and hydrophobic carriers for immobilization were tested, and Duolite A568, which is a hydrophilic resin, was selected as the best carrier. This immobilized lipase was used to synthesize TAG by direct esterification under vacuum. The parameters investigated were temperature, enzyme loading, and vacuum level. The optimum reaction conditions were a temperature of 60 °C, an enzyme loading of 15% (based on the total weight of the substrate), and a vacuum of 0.7 kPa, respectively. The maximum conversion to TAG of ca. 88 wt% was obtained in 12 h under the optimum conditions.

AB - An α-linolenic acid (ALA)-rich triacylglycerol (TAG) was synthesized from an ALA-rich fatty acid (FA) from perilla oil and glycerol, using a newly prepared immobilized lipase under vacuum. The ALA-rich FA (purity >90 wt%) used as the substrate was prepared by urea complexation from perilla oil FAs. Liquid Lipozyme TL 100 L lipase from Thermomyces lanuginosus was used for immobilization. Nine different hydrophilic and hydrophobic carriers for immobilization were tested, and Duolite A568, which is a hydrophilic resin, was selected as the best carrier. This immobilized lipase was used to synthesize TAG by direct esterification under vacuum. The parameters investigated were temperature, enzyme loading, and vacuum level. The optimum reaction conditions were a temperature of 60 °C, an enzyme loading of 15% (based on the total weight of the substrate), and a vacuum of 0.7 kPa, respectively. The maximum conversion to TAG of ca. 88 wt% was obtained in 12 h under the optimum conditions.

KW - Duolite A568

KW - Immobilization

KW - Lipase

KW - Thermomyces lanuginosus

KW - Triacylglycerol

KW - α-Linolenic acid

UR - http://www.scopus.com/inward/record.url?scp=85020252806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020252806&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2017.05.161

DO - 10.1016/j.foodchem.2017.05.161

M3 - Article

C2 - 28764049

AN - SCOPUS:85020252806

VL - 237

SP - 654

EP - 658

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

ER -