Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis

In Cheol Kim, Sang Ho Yoo, Sung-Joon Lee, Byung Ha Oh, Jung Wan Kim, Kwan Hwa Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An E. coli cell extract containing thermostable α-amylase and maltogenic amylase expressed from the clone pTMA322 was used to produce an anomalously linked oligosaccharides (Alo) mixture from starch. The liquefaction and saccharification of starch were done by one-step procedure using the above cell extract. The resulted Alo mixture contained over 40% oligosaccharides having DP 3 or more including branched forms.

Original languageEnglish
Pages (from-to)416-418
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume58
Issue number2
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Bacilli
Amylases
Oligosaccharides
Starch
glucan 1,4-alpha-maltohydrolase
Cell Extracts
Saccharification
Liquefaction
Escherichia coli
Clone Cells
Bacillus licheniformis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis. / Kim, In Cheol; Yoo, Sang Ho; Lee, Sung-Joon; Oh, Byung Ha; Kim, Jung Wan; Park, Kwan Hwa.

In: Bioscience, Biotechnology and Biochemistry, Vol. 58, No. 2, 1994, p. 416-418.

Research output: Contribution to journalArticle

Kim, In Cheol ; Yoo, Sang Ho ; Lee, Sung-Joon ; Oh, Byung Ha ; Kim, Jung Wan ; Park, Kwan Hwa. / Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis. In: Bioscience, Biotechnology and Biochemistry. 1994 ; Vol. 58, No. 2. pp. 416-418.
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