Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions

Ki Wan Bong, Jae Jung Kim, Hansang Cho, Eugene Lim, Patrick S. Doyle, Daniel Irimia

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cell-adhesive particles are of significant interest in biotechnology, the bioengineering of complex tissues, and biomedical research. Their applications range from platforms to increase the efficiency of anchorage-dependent cell culture to building blocks to loading cells in heterogeneous structures to clonal-population growth monitoring to cell sorting. Although useful, currently available cell-adhesive particles can accommodate only homogeneous cell culture. Here, we report the design of anisotropic hydrogel microparticles with tunable cell-adhesive regions as first step toward micropatterned cell cultures on particles. We employed stop flow lithography (SFL), the coupling reaction between amine and N-hydroxysuccinimide (NHS) and streptavidin-biotin chemistry to adjust the localization of conjugated collagen and poly-l-lysine on the surface of microscale particles. Using the new particles, we demonstrate the attachment and formation of tight junctions between brain endothelial cells. We also demonstrate the geometric patterning of breast cancer cells on particles with heterogeneous collagen coatings. This new approach avoids the exposure of cells to potentially toxic photoinitiators and ultraviolet light and decouples in time the microparticle synthesis and the cell culture steps to take advantage of the most recent advances in cell patterning available for traditional culture substrates.

Original languageEnglish
Pages (from-to)13165-13171
Number of pages7
JournalLangmuir
Volume31
Issue number48
DOIs
Publication statusPublished - 2015 Nov 6

Fingerprint

biotin
Streptavidin
Biotin
Cell culture
Adhesives
adhesives
Lithography
lithography
synthesis
cells
Collagen
Cell Culture Techniques
Cells
interactions
microparticles
collagens
Poisons
Hydrogel
Endothelial cells
Biotechnology

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions. / Bong, Ki Wan; Kim, Jae Jung; Cho, Hansang; Lim, Eugene; Doyle, Patrick S.; Irimia, Daniel.

In: Langmuir, Vol. 31, No. 48, 06.11.2015, p. 13165-13171.

Research output: Contribution to journalArticle

Bong, Ki Wan ; Kim, Jae Jung ; Cho, Hansang ; Lim, Eugene ; Doyle, Patrick S. ; Irimia, Daniel. / Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions. In: Langmuir. 2015 ; Vol. 31, No. 48. pp. 13165-13171.
@article{db5c10cea67b403a851471592d6c8fd1,
title = "Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions",
abstract = "Cell-adhesive particles are of significant interest in biotechnology, the bioengineering of complex tissues, and biomedical research. Their applications range from platforms to increase the efficiency of anchorage-dependent cell culture to building blocks to loading cells in heterogeneous structures to clonal-population growth monitoring to cell sorting. Although useful, currently available cell-adhesive particles can accommodate only homogeneous cell culture. Here, we report the design of anisotropic hydrogel microparticles with tunable cell-adhesive regions as first step toward micropatterned cell cultures on particles. We employed stop flow lithography (SFL), the coupling reaction between amine and N-hydroxysuccinimide (NHS) and streptavidin-biotin chemistry to adjust the localization of conjugated collagen and poly-l-lysine on the surface of microscale particles. Using the new particles, we demonstrate the attachment and formation of tight junctions between brain endothelial cells. We also demonstrate the geometric patterning of breast cancer cells on particles with heterogeneous collagen coatings. This new approach avoids the exposure of cells to potentially toxic photoinitiators and ultraviolet light and decouples in time the microparticle synthesis and the cell culture steps to take advantage of the most recent advances in cell patterning available for traditional culture substrates.",
author = "Bong, {Ki Wan} and Kim, {Jae Jung} and Hansang Cho and Eugene Lim and Doyle, {Patrick S.} and Daniel Irimia",
year = "2015",
month = "11",
day = "6",
doi = "10.1021/acs.langmuir.5b03501",
language = "English",
volume = "31",
pages = "13165--13171",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "48",

}

TY - JOUR

T1 - Synthesis of Cell-Adhesive Anisotropic Multifunctional Particles by Stop Flow Lithography and Streptavidin-Biotin Interactions

AU - Bong, Ki Wan

AU - Kim, Jae Jung

AU - Cho, Hansang

AU - Lim, Eugene

AU - Doyle, Patrick S.

AU - Irimia, Daniel

PY - 2015/11/6

Y1 - 2015/11/6

N2 - Cell-adhesive particles are of significant interest in biotechnology, the bioengineering of complex tissues, and biomedical research. Their applications range from platforms to increase the efficiency of anchorage-dependent cell culture to building blocks to loading cells in heterogeneous structures to clonal-population growth monitoring to cell sorting. Although useful, currently available cell-adhesive particles can accommodate only homogeneous cell culture. Here, we report the design of anisotropic hydrogel microparticles with tunable cell-adhesive regions as first step toward micropatterned cell cultures on particles. We employed stop flow lithography (SFL), the coupling reaction between amine and N-hydroxysuccinimide (NHS) and streptavidin-biotin chemistry to adjust the localization of conjugated collagen and poly-l-lysine on the surface of microscale particles. Using the new particles, we demonstrate the attachment and formation of tight junctions between brain endothelial cells. We also demonstrate the geometric patterning of breast cancer cells on particles with heterogeneous collagen coatings. This new approach avoids the exposure of cells to potentially toxic photoinitiators and ultraviolet light and decouples in time the microparticle synthesis and the cell culture steps to take advantage of the most recent advances in cell patterning available for traditional culture substrates.

AB - Cell-adhesive particles are of significant interest in biotechnology, the bioengineering of complex tissues, and biomedical research. Their applications range from platforms to increase the efficiency of anchorage-dependent cell culture to building blocks to loading cells in heterogeneous structures to clonal-population growth monitoring to cell sorting. Although useful, currently available cell-adhesive particles can accommodate only homogeneous cell culture. Here, we report the design of anisotropic hydrogel microparticles with tunable cell-adhesive regions as first step toward micropatterned cell cultures on particles. We employed stop flow lithography (SFL), the coupling reaction between amine and N-hydroxysuccinimide (NHS) and streptavidin-biotin chemistry to adjust the localization of conjugated collagen and poly-l-lysine on the surface of microscale particles. Using the new particles, we demonstrate the attachment and formation of tight junctions between brain endothelial cells. We also demonstrate the geometric patterning of breast cancer cells on particles with heterogeneous collagen coatings. This new approach avoids the exposure of cells to potentially toxic photoinitiators and ultraviolet light and decouples in time the microparticle synthesis and the cell culture steps to take advantage of the most recent advances in cell patterning available for traditional culture substrates.

UR - http://www.scopus.com/inward/record.url?scp=84949655930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949655930&partnerID=8YFLogxK

U2 - 10.1021/acs.langmuir.5b03501

DO - 10.1021/acs.langmuir.5b03501

M3 - Article

VL - 31

SP - 13165

EP - 13171

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 48

ER -