Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017: Utilization as a functional starter culture for milk fermentation

Hyun Chung Soo, In-Hwan Kim, Gyu Park Hui, Soon Kang Hye, Suk Yoon Chil, Yeon Jeong Ha, Jin Choi Nag, Gi Kwon Eung, Jun Kim Young

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study was performed to discover bifidobacteria isolated from human intestines that optimally convert linoleic acid (LA) to conjugated linoleic acid (CLA) and to optimize the culture conditions of milk fermentation. One hundred and fifty neonatal bifidobacteria were screened for CLA-producing ability, and Bifidobacterium breve LMC 017 was selected as it showed about 90% conversion of free LA in MRS broth. The selected strain showed resistance at 0.5% LA in microaerophillic conditions. When monolinolein (LA 90%) was used as a substrate for CLA production, the conversion rate was lower compared to free LA, but the growth rate was unaffected during the milk fermentation. There was no significant difference in CLA production between aerobic and anaerobic conditions, and little decline in CLA was shown after the maximal CLA level had been reached. CLA production increased by 80% with 24 h of incubation in milk containing additional skim milk (5%), where the proteins may have facilitated the production of CLA by enhancing the interaction of substrate with the bacteria. CLA production did not decline after 9 h of fermentation and an additional 12 weeks of storage with other commercial starters. This demonstrates the possibility of using this strain as a costarter in the production of CLA-enriched yogurt.

Original languageEnglish
Pages (from-to)3311-3316
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number9
DOIs
Publication statusPublished - 2008 May 14

Fingerprint

Bifidobacterium breve
Conjugated Linoleic Acids
Starters
starter cultures
conjugated linoleic acid
Fermentation
Milk
fermentation
milk
synthesis
Linoleic Acid
linoleic acid
Bifidobacterium
Yogurt
Milk Proteins
aerobic conditions
Substrates
skim milk
yogurt
anaerobic conditions

Keywords

  • Bifidobacterium breve
  • Conjugated linoleic acid
  • Linoleic acid
  • Milk fermentation
  • Monolinolein

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017 : Utilization as a functional starter culture for milk fermentation. / Soo, Hyun Chung; Kim, In-Hwan; Hui, Gyu Park; Hye, Soon Kang; Chil, Suk Yoon; Ha, Yeon Jeong; Nag, Jin Choi; Eung, Gi Kwon; Young, Jun Kim.

In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 9, 14.05.2008, p. 3311-3316.

Research output: Contribution to journalArticle

Soo, Hyun Chung ; Kim, In-Hwan ; Hui, Gyu Park ; Hye, Soon Kang ; Chil, Suk Yoon ; Ha, Yeon Jeong ; Nag, Jin Choi ; Eung, Gi Kwon ; Young, Jun Kim. / Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017 : Utilization as a functional starter culture for milk fermentation. In: Journal of Agricultural and Food Chemistry. 2008 ; Vol. 56, No. 9. pp. 3311-3316.
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