Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes

Sanjay K.S. Patel, Sachin V. Otari, Jinglin Li, Dong Rip Kim, Sun Chang Kim, Byung Kwan Cho, Vipin C. Kalia, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Herein, we report the preparation of a cross-linked protein-metal hybrid nanoflower (NF) system for laccase immobilization. The immobilized laccase showed effective encapsulation yield and activity recovery of 78.1% and 204%, respectively. The catalytic efficiency (kcat Vmax−1) of cross-linked NF (CL-NF) was 2.2-fold more than that of free laccase. The CL-NF also exhibited significantly higher stability towards pH and temperature changes. It exhibited excellent storage stability and tolerance towards solvents and inhibitors as compared with the free enzyme. After 10 cycles of reuses, the NF and CL-NF laccase showed 41.2% and 92.3% residual activity, respectively. The CL-NF showed high oxidation potential, 265% that of the free enzyme, towards phenolic compounds. The CL-NF laccase retained the residual decolorization efficiency of up to 84.6% for synthetic dyes under repeated batch conditions of 10 cycles. These results suggested that the preparation of CL-NF is an effective approach to enhance the enzymatic properties and has great potential in many industrial applications.

Original languageEnglish
Pages (from-to)442-450
Number of pages9
JournalJournal of hazardous materials
Volume347
DOIs
Publication statusPublished - 2018 Apr 5

Fingerprint

Nanoflowers
Laccase
dye
Coloring Agents
Dyes
Metals
enzyme
Proteins
protein
encapsulation
metal
phenolic compound
Enzymes
immobilization
inhibitor
tolerance
fold
Encapsulation
oxidation
Industrial applications

Keywords

  • Cross-linking
  • Immobilization
  • Laccase
  • Protein-inorganic hybrid
  • Synthetic dyes

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes. / Patel, Sanjay K.S.; Otari, Sachin V.; Li, Jinglin; Kim, Dong Rip; Kim, Sun Chang; Cho, Byung Kwan; Kalia, Vipin C.; Kang, Yun Chan; Lee, Jung Kul.

In: Journal of hazardous materials, Vol. 347, 05.04.2018, p. 442-450.

Research output: Contribution to journalArticle

Patel, Sanjay K.S. ; Otari, Sachin V. ; Li, Jinglin ; Kim, Dong Rip ; Kim, Sun Chang ; Cho, Byung Kwan ; Kalia, Vipin C. ; Kang, Yun Chan ; Lee, Jung Kul. / Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes. In: Journal of hazardous materials. 2018 ; Vol. 347. pp. 442-450.
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