Abstract
Phytosteryl ester synthesized with pinolenic acid (PLA) from pine nut oil is expected to have features of both phytosterol and PLA. In this study, lipase from Candida rugosa (CRL) was immobilized and then used to optimize conditions for synthesis of phytosteryl ester containing PLA. Lewatit VP OC 1600, a macroporous hydrophobic resin, was selected as the best carrier, and the optimum condition for the immobilization of CRL was established. With immobilized CRL prepared, synthesis of phytosteryl ester with fatty acid from pine nut oil was carried out. Parameters investigated were temperature, molar ratio (phytosterol to fatty acid), enzyme loading, and vacuum. Optimum conditions for synthesis of phytosteryl ester were a temperature of 60 C, molar ratio of 1:4, enzyme loading of 10% (based on the total weight of the substrate), and pressure of 80 kPa. The maximum conversion of phytosteryl ester was ca. 93 mol % at the optimum condition.
| Original language | English |
|---|---|
| Pages (from-to) | 8934-8940 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 61 |
| Issue number | 37 |
| DOIs | |
| Publication status | Published - 2013 Sep 18 |
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Keywords
- Candida rugosa
- enzyme immobilization
- phytosteryl ester
- pine nut oil
- pinolenic acid
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
Cite this
Synthesis of phytosteryl ester containing pinolenic acid in a solvent-free system using immobilized Candida rugosa lipase. / No, Da Som; Zhao, Tingting; Lee, Junsoo; Lee, Jeom Sig; Kim, In-Hwan.
In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 37, 18.09.2013, p. 8934-8940.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Synthesis of phytosteryl ester containing pinolenic acid in a solvent-free system using immobilized Candida rugosa lipase
AU - No, Da Som
AU - Zhao, Tingting
AU - Lee, Junsoo
AU - Lee, Jeom Sig
AU - Kim, In-Hwan
PY - 2013/9/18
Y1 - 2013/9/18
N2 - Phytosteryl ester synthesized with pinolenic acid (PLA) from pine nut oil is expected to have features of both phytosterol and PLA. In this study, lipase from Candida rugosa (CRL) was immobilized and then used to optimize conditions for synthesis of phytosteryl ester containing PLA. Lewatit VP OC 1600, a macroporous hydrophobic resin, was selected as the best carrier, and the optimum condition for the immobilization of CRL was established. With immobilized CRL prepared, synthesis of phytosteryl ester with fatty acid from pine nut oil was carried out. Parameters investigated were temperature, molar ratio (phytosterol to fatty acid), enzyme loading, and vacuum. Optimum conditions for synthesis of phytosteryl ester were a temperature of 60 C, molar ratio of 1:4, enzyme loading of 10% (based on the total weight of the substrate), and pressure of 80 kPa. The maximum conversion of phytosteryl ester was ca. 93 mol % at the optimum condition.
AB - Phytosteryl ester synthesized with pinolenic acid (PLA) from pine nut oil is expected to have features of both phytosterol and PLA. In this study, lipase from Candida rugosa (CRL) was immobilized and then used to optimize conditions for synthesis of phytosteryl ester containing PLA. Lewatit VP OC 1600, a macroporous hydrophobic resin, was selected as the best carrier, and the optimum condition for the immobilization of CRL was established. With immobilized CRL prepared, synthesis of phytosteryl ester with fatty acid from pine nut oil was carried out. Parameters investigated were temperature, molar ratio (phytosterol to fatty acid), enzyme loading, and vacuum. Optimum conditions for synthesis of phytosteryl ester were a temperature of 60 C, molar ratio of 1:4, enzyme loading of 10% (based on the total weight of the substrate), and pressure of 80 kPa. The maximum conversion of phytosteryl ester was ca. 93 mol % at the optimum condition.
KW - Candida rugosa
KW - enzyme immobilization
KW - phytosteryl ester
KW - pine nut oil
KW - pinolenic acid
UR - http://www.scopus.com/inward/record.url?scp=84884493635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884493635&partnerID=8YFLogxK
U2 - 10.1021/jf402953n
DO - 10.1021/jf402953n
M3 - Article
C2 - 23988007
AN - SCOPUS:84884493635
VL - 61
SP - 8934
EP - 8940
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 37
ER -