Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]

Vibhor Saraswat, Jeewon Lee, Dae Young Kim, Young Hoon Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using lactose as an inducer, recombinant human interleukin-2 (rhIL-2) was synthesized with an N-terminus fusion partner, G3 (three tandem-arranged glucagon peptides) in fed-batch cultures at high cell concentration (60-90 g l-1) of Escherichia coli BL21(DE3) [pT7-G3IL2]. With batch additions of lactose (4 x 13.5 g), the fusion rhIL-2 was synthesized up to 9.3 g l-1. However, if all the lactose (54 g) was added at once to the culture, synthesized fusion rhIL-2 decreased to 5.4 g l-1 with a decreased cell growth rate. A statistical optimization of the production medium containing glucose, yeast extract, and lactose led to fusion rhIL-2 being produced at > 9 g l-1.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Batch Cell Culture Techniques
Lactose
Escherichia coli
Interleukin-2
Fusion reactions
Cell growth
Glucagon
Yeast
Peptides
Glucose
Yeasts
Growth

Keywords

  • Escherichia coli
  • Interleukin-2
  • Lactose
  • Symport

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]. / Saraswat, Vibhor; Lee, Jeewon; Kim, Dae Young; Park, Young Hoon.

In: Biotechnology Letters, Vol. 22, No. 4, 2000, p. 261-265.

Research output: Contribution to journalArticle

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