Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Niyom Lue; Wonshik Choi; Gabriel Popescu; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1364/OE.16.016240

Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Niyom Lue, Wonshik Choi, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.

Original language	English
Pages (from-to)	16240-16246
Number of pages	7
Journal	Optics Express
Volume	16
Issue number	20
DOIs	https://doi.org/10.1364/OE.16.016240
Publication status	Published - 2008 Sep 29
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

Access to Document

10.1364/OE.16.016240

Cite this

@article{9306e235fff6406a88e7587b9516c119,

title = "Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion",

abstract = "We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.",

author = "Niyom Lue and Wonshik Choi and Gabriel Popescu and Kamran Badizadegan and Dasari, {Ramachandra R.} and Feld, {Michael S.}",

year = "2008",

month = sep,

day = "29",

doi = "10.1364/OE.16.016240",

language = "English",

volume = "16",

pages = "16240--16246",

journal = "Optics Express",

issn = "1094-4087",

publisher = "The Optical Society",

number = "20",

}

TY - JOUR

T1 - Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

AU - Lue, Niyom

AU - Choi, Wonshik

AU - Popescu, Gabriel

AU - Badizadegan, Kamran

AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

PY - 2008/9/29

Y1 - 2008/9/29

N2 - We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.

AB - We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.

UR - http://www.scopus.com/inward/record.url?scp=54749112696&partnerID=8YFLogxK

U2 - 10.1364/OE.16.016240

DO - 10.1364/OE.16.016240

M3 - Article

C2 - 18825263

AN - SCOPUS:54749112696

VL - 16

SP - 16240

EP - 16246

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 20

ER -

Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this