Abstract
We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.
| Original language | English |
|---|---|
| Pages (from-to) | 16240-16246 |
| Number of pages | 7 |
| Journal | Optics Express |
| Volume | 16 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 2008 Sep 29 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion. / Lue, Niyom; Choi, Wonshik; Popescu, Gabriel; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.
In: Optics Express, Vol. 16, No. 20, 29.09.2008, p. 16240-16246.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion
AU - Lue, Niyom
AU - Choi, Wonshik
AU - Popescu, Gabriel
AU - Badizadegan, Kamran
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
PY - 2008/9/29
Y1 - 2008/9/29
N2 - We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.
AB - We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.
UR - http://www.scopus.com/inward/record.url?scp=54749112696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54749112696&partnerID=8YFLogxK
U2 - 10.1364/OE.16.016240
DO - 10.1364/OE.16.016240
M3 - Article
VL - 16
SP - 16240
EP - 16246
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 20
ER -