Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Niyom Lue; Wonshik Choi; Gabriel Popescu; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1364/OE.16.016240

Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Niyom Lue, Wonshik Choi, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.

Original language	English
Pages (from-to)	16240-16246
Number of pages	7
Journal	Optics Express
Volume	16
Issue number	20
DOIs	https://doi.org/10.1364/OE.16.016240
Publication status	Published - 2008 Sep 29
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OE.16.016240

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abstract = "We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.",

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AU - Popescu, Gabriel

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AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

PY - 2008/9/29

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Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Abstract

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