Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion

Niyom Lue, Wonshik Choi, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We present a technique for 3D imaging of live cells in translational motion without need of axial scanning of objective lens. A set of transmitted electric field images of cells at successive points of transverse translation is taken with a focused beam illumination. Based on Hyugens' principle, angular plane waves are synthesized from E-field images of a focused beam. For a set of synthesized angular plane waves, we apply a filtered back-projection algorithm and obtain 3D maps of refractive index of live cells. This technique, which we refer to as synthetic aperture tomographic phase microscopy, can potentially be combined with flow cytometry or microfluidic devices, and will enable high throughput acquisition of quantitative refractive index data from large numbers of cells.

Original languageEnglish
Pages (from-to)16240-16246
Number of pages7
JournalOptics Express
Volume16
Issue number20
DOIs
Publication statusPublished - 2008 Sep 29
Externally publishedYes

Fingerprint

synthetic apertures
translational motion
microscopy
cells
plane waves
refractivity
cytometry
microfluidic devices
acquisition
projection
illumination
lenses
scanning
electric fields

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion. / Lue, Niyom; Choi, Wonshik; Popescu, Gabriel; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.

In: Optics Express, Vol. 16, No. 20, 29.09.2008, p. 16240-16246.

Research output: Contribution to journalArticle

Lue, Niyom ; Choi, Wonshik ; Popescu, Gabriel ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. / Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion. In: Optics Express. 2008 ; Vol. 16, No. 20. pp. 16240-16246.
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