Syringaresinol induces mitochondrial biogenesis through activation of PPARβ pathway in skeletal muscle cells

Trung Thanh Thach, Chan Kyu Lee, Hyun woo Park, Sang Jun Lee, Sung-Joon Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Activation of peroxisome proliferator-activated receptors (PPARs) plays a crucial role in cellular energy metabolism that directly impacts mitochondrial biogenesis. In this study, we demonstrate that syringaresinol, a pharmacological lignan extracted from Panax ginseng berry, moderately binds to and activates PPARβ with KD and EC50 values of 27.62 ± 15.76 μM and 18.11 ± 4.77 μM, respectively. Subsequently, the expression of peroxisome proliferator-activated receptor γ coactivator-1α together with PPARβ transcriptional targets, mitochondrial carnitine palmitoyltransferase 1 and uncoupling protein 2, was also enhanced in terms of both mRNA and protein levels. The activation of these proteins induced mitochondrial biogenesis by enrichment of mitochondrial replication and density within C2C12 myotubes. Importantly, knockdown of PPARβ reduced the syringaresinol-induced protein expression followed by the significant reduction of mitochondrial biogenesis. Taken together, our results indicate that syringaresinol induces mitochondrial biogenesis by activating PPARβ pathway.

Original languageEnglish
Pages (from-to)3978-3983
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume26
Issue number16
DOIs
Publication statusPublished - 2016 Aug 15

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Keywords

  • Mitochondrial biogenesis
  • PPARβ
  • Skeletal muscle
  • Syringaresinol

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

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